Document Detail


Use of an electrostatic dust cloth for self-administered home allergen collection.
MedLine Citation:
PMID:  18361715     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Most epidemiologic studies employ a vacuum cleaner used by a trained technician to collect household allergens. This approach is labor intensive, equipment dependent, and impractical if study subjects reside over a wide geographic area. We examined the feasibility of a self-administered dust collection method, using an electrostatic cloth sent by conventional mail, to obtain allergen measurements. Thirty-two nonasthmatic twins from the California Twin Program wiped areas in the family room, kitchen, and bedroom, according to standardized instructions, and returned the cloths by mail. Allergen concentrations for Der-p-1, Der-f-1, Fel-d-1, and Bla-g-2 were determined using ELISA, and intrahouse and room-to-room concentrations were compared. Der-p-1 and Fel-d-1 were found in most homes, with highest concentrations in bedrooms and kitchens, respectively. Der-f-1 and Bla-g-2 were rarely found. Intrahouse Der-p-1 and Fel-d-1 concentrations were highly correlated and statistically significant (for Der-p-1, bedroom vs. kitchen, p=.0003, bedroom vs. family room, p=.0001, and family room vs. kitchen, p=.002; for Fel-d-1, bedroom vs. kitchen, p=.0004, bedroom vs. family room, p<.0001, and family room vs. kitchen, p=.0001). Reported cat ownership was strongly correlated with household Fel-d-1 concentrations (p<.005). In another comparison from different homes of children enrolled in the La Casa atopy prevention study, allergen concentrations measured from dust collected by a single operator from the left and right half of the same room in 21 homes were compared. Levels of Bla-g-2, Der-p-1, and Fel-d-1 concentrations collected from right and left halves of the same room were highly correlated, with r2 ranging from .7 to .9, and were highly statistically significant (all p values<.01). We conclude that nonintrusive and self-administered dust collection, using commercially available electrostatic dust cloths, sent by conventional mail services, is a promising alternative to technician-collected vacuumed dust for measuring indoor allergens in population-based studies, although further validation of the method is necessary.
Authors:
Wendy Cozen; Ed Avol; David Diaz-Sanchez; Rob McConnell; W James Gauderman; Myles G Cockburn; John Zadnick; Minna Jyrala; Thomas M Mack
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Twin Study    
Journal Detail:
Title:  Twin research and human genetics : the official journal of the International Society for Twin Studies     Volume:  11     ISSN:  1832-4274     ISO Abbreviation:  -     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-03-25     Completed Date:  2008-09-04     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  101244624     Medline TA:  Twin Res Hum Genet     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  150-5     Citation Subset:  IM    
Affiliation:
Department of Preventive Medicine, Keck School of Medicine, University of Southern California, Los Angeles, California 90089-9175, United States of America. wcozen@usc.edu
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MeSH Terms
Descriptor/Qualifier:
Air Pollution, Indoor / analysis*
Allergens / analysis*
Animals
Antigens, Dermatophagoides / analysis
Aspartic Acid Endopeptidases / analysis
California
Cats
Cockroaches
Dust*
Enzyme-Linked Immunosorbent Assay
Feasibility Studies
Glycoproteins / analysis
Housing*
Humans
Mites
Questionnaires
Textiles*
Grant Support
ID/Acronym/Agency:
5P30 ES07048/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/Allergens; 0/Antigens, Dermatophagoides; 0/Dust; 0/Fel d 1 protein, Felis domesticus; 0/Glycoproteins; EC 3.4.22.-/Dermatophagoides farinae antigen f 1; EC 3.4.22.-/Dermatophagoides pteronyssinus antigen p 1; EC 3.4.23.-/Aspartic Acid Endopeptidases; EC 3.4.23.-/allergen Bla g 2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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