Document Detail


Use of electively cryopreserved microsurgically aspirated epididymal sperm with IVF and intracytoplasmic sperm injection for obstructive azoospermia.
MedLine Citation:
PMID:  11020509     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To investigate the efficacy of using intentionally cryopreserved epididymal sperm in selected cases of obstructive azoospermia. DESIGN: A retrospective, nonrandomized study. SETTING: Academic research environment. PATIENTS: One hundred forty-one couples undergoing first-time IVF/ICSI using either fresh or cryopreserved epididymal sperm. INTERVENTIONS: The epididymides were microsurgically aspirated. MAIN OUTCOME MEASURES: Clinical pregnancy rates. RESULTS: Motile sperm were obtained from all men. For the fresh group, the mean total sperm aspirated was 99 x 10(6) with 5.5 vials frozen per patient after ICSI and 82 x 10(6) with 4.7 vials frozen per patient in the cryopreserved group. No statistically significant difference in oocyte fertilization rate or number of embryos transferred was noted between groups. Of 108 patients using freshly aspirated sperm, 72 (66.7%) achieved clinical pregnancy. Of 33 patients in the group using cryopreserved sperm, 20 (60.6%) achieved clinical pregnancy (P=0.47). CONCLUSIONS: In selected ideal cases of unreconstructable azoospermia, elective open microsurgical epididymal sperm aspiration with cryopreservation yields pregnancy rates similar to that employing fresh sperm. The advantages of this method are: (1) Use of cryopreserved sperm obviates the logistics problems associated with the use of fresh sperm. (2) Abundant high-quality sperm can be cryopreserved in a single procedure for all future attempts at IVF/ICSI. Rarely, viable sperm will not be present after thawing, and fresh retrieval will be necessary.
Authors:
N Janzen; M Goldstein; P N Schlegel; G D Palermo; Z Rosenwaks; J Hariprashad
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Fertility and sterility     Volume:  74     ISSN:  0015-0282     ISO Abbreviation:  Fertil. Steril.     Publication Date:  2000 Oct 
Date Detail:
Created Date:  2000-11-29     Completed Date:  2000-11-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372772     Medline TA:  Fertil Steril     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  696-701     Citation Subset:  IM    
Affiliation:
The Cornell Institute for Reproductive Medicine, The New York Weill Cornell Medical Center, New York, New York, USA.
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MeSH Terms
Descriptor/Qualifier:
Cryopreservation
Epididymis
Female
Fertilization in Vitro*
Humans
Male
Microsurgery
Oligospermia*
Pregnancy
Pregnancy Outcome
Semen Preservation*
Sperm Injections, Intracytoplasmic*
Sperm Motility
Comments/Corrections
Erratum In:
Fertil Steril 2001 Jan;75(1):230

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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