| Use of the dried blood spot sampling process coupled with fast gas chromatography and negative-ion chemical ionization tandem mass spectrometry: application to fluoxetine, norfluoxetine, reboxetine, and paroxetine analysis. | |
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MedLine Citation:
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PMID: 20063149 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The objective of this work was to combine the advantages of the dried blood spot (DBS) sampling process with the highly sensitive and selective negative-ion chemical ionization tandem mass spectrometry (NICI-MS-MS) to analyze for recent antidepressants including fluoxetine, norfluoxetine, reboxetine, and paroxetine from micro whole blood samples (i.e., 10 microL). Before analysis, DBS samples were punched out, and antidepressants were simultaneously extracted and derivatized in a single step by use of pentafluoropropionic acid anhydride and 0.02% triethylamine in butyl chloride for 30 min at 60 degrees C under ultrasonication. Derivatives were then separated on a gas chromatograph coupled with a triple-quadrupole mass spectrometer operating in negative selected reaction monitoring mode for a total run time of 5 min. To establish the validity of the method, trueness, precision, and selectivity were determined on the basis of the guidelines of the "Soci?t? Fran?aise des Sciences et des Techniques Pharmaceutiques" (SFSTP). The assay was found to be linear in the concentration ranges 1 to 500 ng mL(-1) for fluoxetine and norfluoxetine and 20 to 500 ng mL(-1) for reboxetine and paroxetine. Despite the small sampling volume, the limit of detection was estimated at 20 pg mL(-1) for all the analytes. The stability of DBS was also evaluated at -20 degrees C, 4 degrees C, 25 degrees C, and 40 degrees C for up to 30 days. Furthermore, the method was successfully applied to a pharmacokinetic investigation performed on a healthy volunteer after oral administration of a single 40-mg dose of fluoxetine. Thus, this validated DBS method combines an extractive-derivative single step with a fast and sensitive GC-NICI-MS-MS technique. Using microliter blood samples, this procedure offers a patient-friendly tool in many biomedical fields such as checking treatment adherence, therapeutic drug monitoring, toxicological analyses, or pharmacokinetic studies. |
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Authors:
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Julien D?glon; Estelle Lauer; Aur?lien Thomas; Patrice Mangin; Christian Staub |
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Publication Detail:
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Type: Journal Article Date: 2010-01-10 |
Journal Detail:
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Title: Analytical and bioanalytical chemistry Volume: 396 ISSN: 1618-2650 ISO Abbreviation: Anal Bioanal Chem Publication Date: 2010 Apr |
Date Detail:
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Created Date: 2010-03-19 Completed Date: 2010-06-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101134327 Medline TA: Anal Bioanal Chem Country: Germany |
Other Details:
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Languages: eng Pagination: 2523-32 Citation Subset: IM |
Affiliation:
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Unit of Toxicology, University Center of Legal Medicine, 1 rue Michel-Servet, 1211 Geneva 4, Switzerland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anions Antidepressive Agents / blood* Blood Chemical Analysis / methods* Blood Specimen Collection / methods Desiccation Gas Chromatography-Mass Spectrometry / methods* Humans Reproducibility of Results Sensitivity and Specificity Serotonin Uptake Inhibitors / blood* Spectrometry, Mass, Electrospray Ionization / methods* Street Drugs / blood* Substance Abuse Detection / methods* |
| Chemical | |
Reg. No./Substance:
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0/Anions; 0/Antidepressive Agents; 0/Serotonin Uptake Inhibitors; 0/Street Drugs |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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