| Use of deuterium labelled glucose in evaluating the pathway of hepatic glycogen synthesis. | |
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MedLine Citation:
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PMID: 2930527 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Deuterium labelled glucose has been used to study the pathway of hepatic glycogen synthesis during the fasted-refed transition in rats. Deuterium enrichment of liver glycogen was determined using nuclear magnetic resonance as well as mass spectroscopy. Sixty minutes after oral administration of deuterated glucose to fasted rats, the portal vein blood was fully enriched with deuterated glucose. Despite this, less than half of the glucose molecules incorporated into liver glycogen contained deuterium. The loss of deuterium label from glucose is consistent with hepatic glycogen synthesis by an indirect pathway requiring prior metabolism of glucose. The use of deuterium labelled glucose may prove to be a useful probe to study hepatic glycogen metabolism. Its use may also find application in the study of liver glycogen metabolism in humans by a noninvasive means. |
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Authors:
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M N Goodman; L K Masuoka; J S deRopp; A D Jones |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 159 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1989 Mar |
Date Detail:
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Created Date: 1989-04-25 Completed Date: 1989-04-25 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 522-7 Citation Subset: IM |
Affiliation:
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Department of Medicine, Davis School of Medicine, Sacramento, CA 95817. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Glucose / metabolism Deuterium / diagnostic use* Glucose / diagnostic use* Glycogen / biosynthesis* Liver / metabolism* Magnetic Resonance Spectroscopy Male Models, Biological Rats Rats, Inbred Strains |
| Grant Support | |
ID/Acronym/Agency:
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DK 38740/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Blood Glucose; 50-99-7/Glucose; 7782-39-0/Deuterium; 9005-79-2/Glycogen |
| Comments/Corrections | |
Erratum In:
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Biochem Biophys Res Commun 1989 Jul 14;162(1):557 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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