Document Detail


Use of the cytokinesis-block method for the analysis of micronuclei in V79 Chinese hamster lung cells: results with mitomycin C and cyclophosphamide.
MedLine Citation:
PMID:  2492079     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cytochalasin B (CYB)-blocked binucleated cell assay has been explored to analyze micronuclei and cell cycle kinetics using 2 known mutagenic carcinogens in V79 Chinese hamster lung cells. To determine the optimum time to obtain the maximum number of binucleated cells for micronucleus analysis, duplicate cultures of exponentially growing cells were treated with 3 micrograms/ml CYB for varying durations (8-48 h). A peak appearance of binucleated cells at 16 h in the presence of CYB suggested this as an optimum time for micronucleus analysis in binucleated V79 cells. To evaluate the capacity for induction of micronuclei in V79 cells, 2 mutagenic carcinogens, mitomycin C (0.125-1.0 micrograms/ml) and cyclophosphamide (2-12 micrograms/ml) were tested in duplicate cultures. Mitomycin C, a direct-acting alkylating agent, caused approximately an 18-fold increase in micronucleus frequency over controls at the highest concentration tested (1.0 micrograms/ml), and this increase occurred in a dose-related manner (r = 0.92). The concentrations of mitomycin C tested also caused a significant dose-related cell cycle delay, thus suggesting cytotoxicity to V79 cells. Cyclophosphamide, an indirect-acting alkylating agent, requiring the presence of S9 mix, caused approximately a 17-fold increase in micronucleus frequency over controls at the highest tested concentration (12 micrograms/ml), with a clear dose response (r = 0.99). The various concentrations of cyclophosphamide also caused cytotoxicity in a dose-related fashion. Thus, this study demonstrates the usefulness of the cytokinesis-block method in V79 cells as a possible screen to analyze micronucleus induction and cytotoxicity. Because this approach is much less labor intensive than conducting a structural chromosomal analysis, this assay has great potential both as an initial screen for clastogenic activity and as a tool for investigating the underlying mechanisms for clastogenicity.
Authors:
G Krishna; M L Kropko; J C Theiss
Related Documents :
21967759 - Collagen intermingled chitosan-tripolyphosphate nano/micro fibrous scaffolds for tissue...
3489219 - Cellular uptake and cell-associated activity of third generation cephalosporins.
2044929 - Proliferation of endocrine cells in the rat stomach caused by drug-induced achlorhydria.
20423229 - Enhancement of radiosensitivity by cpg-oligodeoxyribonucleotide-7909 in human non-small...
19197989 - Areca nut-induced micronuclei and cytokinesis failure in chinese hamster ovary cells is...
20556539 - A basic study on the biological monitoring for vanadium-effects of vanadium on vero cel...
1381709 - Receptor phenotype underlies differential response of hepatocytes and nonparenchymal ce...
17011159 - Berberine induces apoptosis through a mitochondrial/caspase pathway in human promonocyt...
21979569 - Susceptibility to acmnpv and expression of recombinant proteins by a novel cell clone d...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Mutation research     Volume:  222     ISSN:  0027-5107     ISO Abbreviation:  Mutat. Res.     Publication Date:  1989 Jan 
Date Detail:
Created Date:  1989-02-16     Completed Date:  1989-02-16     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0400763     Medline TA:  Mutat Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  63-9     Citation Subset:  IM    
Affiliation:
Department of Pathology and Experimental Toxicology, Parke-Davis Pharmaceutical Research Division, Warner-Lambert Company, Ann Arbor, MI 48105.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Biotransformation
Cell Cycle / drug effects
Cell Line
Cricetinae
Cricetulus
Cyclophosphamide / pharmacology*
Cytochalasin B / pharmacology
Lung
Male
Micronucleus Tests*
Microsomes, Liver / metabolism
Mitomycin
Mitomycins / pharmacology*
Mutagens*
Rats
Rats, Inbred Strains
Regression Analysis
Chemical
Reg. No./Substance:
0/Mitomycins; 0/Mutagens; 14930-96-2/Cytochalasin B; 50-07-7/Mitomycin; 50-18-0/Cyclophosphamide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Characterization of aminoalkylimidazol-4-one mutagens from liquid-reflux models.
Next Document:  Molecular cloning, genomic structure and localization in a blood stage antigen of Plasmodium falcipa...