| Use of a custom array to study differentially expressed genes during blood orange (Citrus sinensis L. Osbeck) ripening. | |
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MedLine Citation:
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PMID: 19864041 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A flesh-specific oligonucleotide custom array was designed to study gene expression during blood orange ripening. The array included 301 probes derived from a subtracted SSH library, a cDNA-AFLP collection, and a set of regulatory genes from the Harvest citrus database. The custom array was hybridized using samples of Moro, a pigmented cultivar, and Cadenera, a common cultivar, at three different ripening stages: the immature phase, the halfway point of maturation (corresponding to the start of Moro pigmentation) and the full ripening. Of the 301 probes, 27 in total, corresponding to 20 different transcripts, indicated differential expression in stage-to-stage and/or cultivar-to-cultivar comparisons. Transcripts encoding for anthocyanin biosynthesis represented most of the total over-expressed probes. The remaining differentially expressed transcripts were functionally associated with primary metabolism as flavor biosynthesis, defense and signal transduction. The expressed products associated with probes indicating differential expression were confirmed by qRT-PCR. The microarray was designed considering a small collection of sequences useful for monitoring specific pathways and regulatory genes related to fruit ripening and anthocyanin pigmentation. The main novelty of this customization is the use of expressed sequences specifically derived from blood orange flesh to study different cultivars and ripening stages, and the provision of further information about processes related to anthocyanin pigmentation in citrus fruit flesh. |
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Authors:
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Jamila Bernardi; Concetta Licciardello; Maria Patrizia Russo; Maria Luisa Chiusano; Giorgia Carletti; Giuseppe Reforgiato Recupero; Adriano Marocco |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-10-27 |
Journal Detail:
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Title: Journal of plant physiology Volume: 167 ISSN: 1618-1328 ISO Abbreviation: J. Plant Physiol. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-02-09 Completed Date: 2010-05-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9882059 Medline TA: J Plant Physiol Country: Germany |
Other Details:
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Languages: eng Pagination: 301-10 Citation Subset: IM |
Copyright Information:
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Copyright 2009 Elsevier GmbH. All rights reserved. |
Affiliation:
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Istituto di Agronomia, Genetica e Coltivazioni erbacee, Universit? Cattolica del Sacro Cuore, via Emilia Parmense 84, 29100 Piacenza, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Citrus sinensis
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genetics*,
growth & development* Cluster Analysis Expressed Sequence Tags Fruit / genetics*, growth & development* Gene Expression Profiling / methods* Gene Expression Regulation, Plant* Oligonucleotide Array Sequence Analysis / methods* Pigmentation / genetics Reverse Transcriptase Polymerase Chain Reaction Solubility |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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