Document Detail

Use of cultured epithelia to study transport and its regulation.
MedLine Citation:
PMID:  6317789     Owner:  NLM     Status:  MEDLINE    
Epithelial cells from a variety of species and organs form polarized epithelia in culture. When epithelia are grown on a porous surface, such as a millipore filter, transport can be studied using adaptations of standard techniques. In the few years in which cultured epithelia have been studied by transport physiologists, most work has been focused on identification and description of the differentiated transport exhibited by cultured epithelia. Epithelia formed by a continuous line of cells derived from pig kidney (LLC-PK1) exhibit sodium-coupled glucose transport similar to that of the proximal tubule and have vasopressin-sensitive adenylate cyclase that has been studied in great detail. Also of interest are epithelia formed by continuous lines of cells derived from amphibian kidney (A6) and from amphibian urinary bladder (TBM). Each line forms epithelia that have high electrical resistance and amiloride-sensitive sodium transport. Transport is stimulated by aldosterone and by cAMP or hormones that raise cell cAMP levels. In LLC-PK1 and in A6 epithelia, transport and the response to hormones can be manipulated by manipulating the culture conditions. Cultured epithelia have also been used to explore the cell biology of epithelia. Most interesting in this regard are studies of the development and maintenance of epithelial cell polarity. This approach should be especially valuable.
J S Handler
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Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  The Journal of experimental biology     Volume:  106     ISSN:  0022-0949     ISO Abbreviation:  J. Exp. Biol.     Publication Date:  1983 Sep 
Date Detail:
Created Date:  1984-02-22     Completed Date:  1984-02-22     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0243705     Medline TA:  J Exp Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  55-69     Citation Subset:  IM    
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MeSH Terms
Aldosterone / physiology
Biological Transport
Cell Line
Citrate (si)-Synthase
Culture Techniques
Epithelial Cells
Epithelium / physiology*
Intercellular Junctions / physiology
Kidney / physiology
Membrane Potentials
Sodium-Potassium-Exchanging ATPase / metabolism
Urinary Bladder / physiology
Water-Electrolyte Balance*
Reg. No./Substance:
52-39-1/Aldosterone; EC (si)-Synthase; EC ATPase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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