| Use of culture-independent analysis to reveal alteration of intestinal microflora by heat-killed Lactobacillus pentosus in a mouse model of endogenous sepsis. | |
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MedLine Citation:
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PMID: 23277389 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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In this study we evaluated alteration of intestinal microflora by terminal-restriction fragment length polymorphism (T-RFLP) analysis and quantitative PCR (qPCR) for specific microbes. The effects of orally administered heat-killed Lactobacillus pentosus strain b240 (HK-b240) in immunosuppressed mice with endogenous Pseudomonas aeruginosa sepsis was estimated. By T-RFLP analysis, 5 dominant operational taxonomic units (OTUs) including Bacteroides spp. (OTU460) and Lactobacillus spp. (OTU657) were consistently observed, irrespective of treatment, at all time points. A significantly higher population of segmented filamentous bacteria (SFB) was observed by qPCR after 3 weeks of HK-b240 administration; thereafter, the difference was not sustained during immunosuppression and progression of sepsis. Although not significant, Lactobacillus spp. accounted for a larger population in the HK-b240-treated group. In conclusion, this study demonstrated successful application of culture-independent assays for evaluating biological agents by detecting changes in microflora even if the protection was not sufficient to result in significant survival change. |
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Authors:
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Yasuyuki Sugasawa; Tomoo Saga; Soichiro Kimura; Yoshikazu Ishii; Keizo Yamaguchi; Kazuhiro Tateda |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2013-1-1 |
Journal Detail:
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Title: Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy Volume: - ISSN: 1437-7780 ISO Abbreviation: J. Infect. Chemother. Publication Date: 2013 Jan |
Date Detail:
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Created Date: 2013-1-1 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9608375 Medline TA: J Infect Chemother Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Microbiology and Infectious Diseases, Toho University School of Medicine, 5-21-16 Omori-nishi, Ota-ku, Tokyo, 143-8540, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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