Document Detail


Use of the cross-section technique linked with multivariate calibration methods to resolve complex pesticide mixtures.
MedLine Citation:
PMID:  10617427     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The potential of the cross-section (CS) approach in combination with the partial least squares (PLS) and principal component regression (PCR) was assessed in the resolution of a complex pesticide mixture showing twelve overlapped components in High Performance Liquid Chromatography with Diode Array Detection (HPLC-DAD). Careful selection of the CS through the three-dimensional (3D) (A, lambda, t) data matrix gave two-dimensional (2D) signals with the best sensitivity for the determination of each pesticide. In all cases, the application of the PLS method demonstrated a better quantitative prediction ability than that of the PCR method. The CS-PLS approach is a powerful analytical tool. Ten pesticides were well-resolved, while for the other two pesticides of the mixture prediction ability was poor, and they could not be determined, probably due to their low net analytical signal. The CS-PLS model was evaluated by predicting the concentrations of independent test set samples. Finally, the proposed model was successfully applied for the determination of these pesticides in groundwater.
Authors:
A G Frenich; J Martínez Vidal; M Martínez Galera
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical chemistry     Volume:  71     ISSN:  0003-2700     ISO Abbreviation:  Anal. Chem.     Publication Date:  1999 Nov 
Date Detail:
Created Date:  1999-12-27     Completed Date:  1999-12-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4844-50     Citation Subset:  IM    
Affiliation:
Department of Hydrogeology and Analytical Chemistry, University of Almeria, Spain.
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MeSH Terms
Descriptor/Qualifier:
Calibration
Chromatography, High Pressure Liquid
Least-Squares Analysis
Mathematical Computing
Multivariate Analysis
Pesticides / analysis*
Polymerase Chain Reaction
Sensitivity and Specificity
Software
Water Pollutants, Chemical / analysis*
Water Supply / analysis
Chemical
Reg. No./Substance:
0/Pesticides; 0/Water Pollutants, Chemical

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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