| Use of a commercial line blot assay as a screening test for autoantibodies in inflammatory myopathies. | |
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MedLine Citation:
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PMID: 19285154 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIMS: To evaluate the clinical utility of a commercial immunoblot assay for the detection of myositis-specific autoantibodies. METHODS: Serum samples from 153 myositis patients and 77 disease controls were investigated. The commercial Euroline assay with seven autoantigens (Mi-2, Ku, PM-Scl, Jo-1, Pl-7, Pl-12 and SSA/Ro-52) was used according to the manufacturer s instructions, and supplemented with an anti-SRP strip. In a separate experiment analyses were performed at different temperatures. Results were recorded with densitometry. RESULTS: Anti-Jo-1 was found in 18 myositis and one systemic sclerosis patient. Antibodies against Mi-2 were found in 5 myositis patients, and eleven myositis patients had antibodies against PM-Scl. Four myositis patients showed anti-Pl-7 reactivity, whereas no patients had antibodies against Pl-12. Anti-Ku antibodies were found in 4 myositis and 2 primary Sj?gren's syndrome patients. Anti-SRP was found in 8 myositis patients as well as in two disease controls. Antibodies against SSA/Ro52 ranged between 23-62% in all groups except juvenile dermatomyositis patients. Most autoantibody reactivities were clearly positive, only 11% (14/127) were borderline positive. Higher assay temperature increased antibody reactivities. CONCLUSIONS: Except for anti-SSA/Ro-52 and anti-Ku the antibody reactivities were rather myositis-specific, supporting the use of this immunoblot assay. However, assay validation needs to be determined against other methods. |
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Authors:
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Johan R?nnelid; Sevim Barbasso Helmers; Helena Storfors; Katarina Grip; Lars R?nnblom; Karin Franck-Larsson; Gunnel Nordmark; Ingrid E Lundberg |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-03-11 |
Journal Detail:
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Title: Autoimmunity reviews Volume: 9 ISSN: 1873-0183 ISO Abbreviation: Autoimmun Rev Publication Date: 2009 Sep |
Date Detail:
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Created Date: 2009-09-11 Completed Date: 2010-02-25 Revised Date: 2010-03-29 |
Medline Journal Info:
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Nlm Unique ID: 101128967 Medline TA: Autoimmun Rev Country: Netherlands |
Other Details:
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Languages: eng Pagination: 58-61 Citation Subset: IM |
Affiliation:
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Unit of Clinical Immunology, Uppsala University and Uppsala University Hospital, SE-75185 Uppsala, Sweden. johan.ronnelid@klinimm.uu.se |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Aged Aged, 80 and over Autoantibodies / blood Autoantigens / immunology* Feasibility Studies Female Humans Immunoblotting* Lupus Erythematosus, Systemic / blood, diagnosis, immunology Male Middle Aged Myositis / blood, diagnosis*, immunology Scleroderma, Systemic / blood, diagnosis, immunology Sensitivity and Specificity Temperature |
| Chemical | |
Reg. No./Substance:
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0/Autoantibodies; 0/Autoantigens |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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