| Use of cellulase inhibitors to produce cellobiose. | |
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MedLine Citation:
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PMID: 20703956 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The economics driving biorefinery development requires high value-added products such as cellobiose for financial feasibility. This research describes a simple technology for increasing cellobiose yields during lignocellulosic hydrolysis. The yield of cellobiose produced during cellulose hydrolysis was maximized by modification of reaction conditions. The addition of an inhibitor from the group that includes glucose oxidase, gluconolactone, and gluconic acid during cellulase hydrolysis of cellulose increased the amount of cellobiose produced. The optimal conditions for cellobiose production were determined for four factors; reaction time, cellulase concentration, cellulose concentration, and inhibitor concentration using a Box-Behnken experimental design. Gluconolactone in the cellulase system resulted in the greatest production of cellobiose (31.2%) from cellulose. The yield of cellobiose was 23.7% with glucose oxidase, similar to 21.9% with gluconic acid. |
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Authors:
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Misook Kim; Donal F Day |
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Publication Detail:
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Type: Journal Article Date: 2010-02-21 |
Journal Detail:
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Title: Applied biochemistry and biotechnology Volume: 162 ISSN: 1559-0291 ISO Abbreviation: Appl. Biochem. Biotechnol. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-08-12 Completed Date: 2010-11-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8208561 Medline TA: Appl Biochem Biotechnol Country: United States |
Other Details:
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Languages: eng Pagination: 1379-90 Citation Subset: IM |
Affiliation:
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Audubon Sugar Institute, St. Gabriel, LA 70776, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Analysis of Variance Biomass Cellobiose / biosynthesis* Cellulase / antagonists & inhibitors*, metabolism Cellulose Enzyme Inhibitors / pharmacology* Gluconates / pharmacology Glucose Oxidase / metabolism Hydrolysis / drug effects Kinetics Lactones / pharmacology Lignin / metabolism Trichoderma / enzymology* |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Inhibitors; 0/Gluconates; 0/Lactones; 11132-73-3/lignocellulose; 16462-44-5/Cellobiose; 526-95-4/gluconic acid; 90-80-2/beta-glucono-1,5-lactone; 9004-34-6/Cellulose; 9005-53-2/Lignin; 9006-97-7/bagasse; EC 1.1.3.4/Glucose Oxidase; EC 3.2.1.4/Cellulase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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