Document Detail

Use of cellulase inhibitors to produce cellobiose.
MedLine Citation:
PMID:  20703956     Owner:  NLM     Status:  MEDLINE    
The economics driving biorefinery development requires high value-added products such as cellobiose for financial feasibility. This research describes a simple technology for increasing cellobiose yields during lignocellulosic hydrolysis. The yield of cellobiose produced during cellulose hydrolysis was maximized by modification of reaction conditions. The addition of an inhibitor from the group that includes glucose oxidase, gluconolactone, and gluconic acid during cellulase hydrolysis of cellulose increased the amount of cellobiose produced. The optimal conditions for cellobiose production were determined for four factors; reaction time, cellulase concentration, cellulose concentration, and inhibitor concentration using a Box-Behnken experimental design. Gluconolactone in the cellulase system resulted in the greatest production of cellobiose (31.2%) from cellulose. The yield of cellobiose was 23.7% with glucose oxidase, similar to 21.9% with gluconic acid.
Misook Kim; Donal F Day
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Publication Detail:
Type:  Journal Article     Date:  2010-02-21
Journal Detail:
Title:  Applied biochemistry and biotechnology     Volume:  162     ISSN:  1559-0291     ISO Abbreviation:  Appl. Biochem. Biotechnol.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-08-12     Completed Date:  2010-11-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8208561     Medline TA:  Appl Biochem Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1379-90     Citation Subset:  IM    
Audubon Sugar Institute, St. Gabriel, LA 70776, USA.
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MeSH Terms
Analysis of Variance
Cellobiose / biosynthesis*
Cellulase / antagonists & inhibitors*,  metabolism
Enzyme Inhibitors / pharmacology*
Gluconates / pharmacology
Glucose Oxidase / metabolism
Hydrolysis / drug effects
Lactones / pharmacology
Lignin / metabolism
Trichoderma / enzymology*
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Gluconates; 0/Lactones; 11132-73-3/lignocellulose; 16462-44-5/Cellobiose; 526-95-4/gluconic acid; 90-80-2/beta-glucono-1,5-lactone; 9004-34-6/Cellulose; 9005-53-2/Lignin; 9006-97-7/bagasse; EC Oxidase; EC

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