| USE OF CALCIUM DIPICOLINATE FOR ENUMERATION OF TOTAL VIABLE ENDOSPORE POPULATIONS WITHOUT HEAT ACTIVATION. | |
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MedLine Citation:
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PMID: 14131356 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A method, based on germination with 50 mm CaCl(2) and 40 mm sodium dipicolinate (Na(2)DPA), was developed for the determination of total viable counts of bacterial spores requiring heat activation. Incorporation of these germinating agents into Tryptone Glucose Extract Agar permitted plate-count enumeration of essentially 100% of Bacillus subtilis strain 5230 spores without a heat treatment. Other spore suspensions were surveyed for their response to CaCl(2) and Na(2)DPA, and for the subsequent removal of the heat-activation requirement for enumeration of maximal populations. |
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Authors:
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F F BUSTA; Z J ORDAL |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Applied microbiology Volume: 12 ISSN: 0003-6919 ISO Abbreviation: Appl Microbiol Publication Date: 1964 Mar |
Date Detail:
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Created Date: 1964-07-01 Completed Date: 1996-12-01 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7605802 Medline TA: Appl Microbiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 106-10 Citation Subset: OM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacillus* Bacillus megaterium* Bacillus subtilis* Calcium* Hot Temperature* Pyridines* Research* |
| Chemical | |
Reg. No./Substance:
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0/Pyridines; 7440-70-2/Calcium |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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