Document Detail

Use of broad-spectrum antimicrobials in the eradication of unknown aquatic pathogens in a zebrafish larval rearing system.
MedLine Citation:
PMID:  15773777     Owner:  NLM     Status:  MEDLINE    
A zebrafish larval rearing system experienced a surge in mortality rates soon after the introduction of new stocks. A comprehensive water analysis of pH, nitrites, nitrates, ammonia, chlorine, carbonate hardness, general hardness, and conductivity identified no anomalies. Observations via light microscopy of affected fry revealed consistent signs of impaired mobility, blood clotting, and eventual heart hemorrhage resulting in the death of 90 to 100% of the fry by the age of 2 weeks. Collection of sufficient tissue samples for a histological investigation proved problematic due to the fry's diminutive size. Because a causal agent could not be isolated satisfactorily, the use of a broad-spectrum antibiotic was deemed necessary. After considering many broad-spectrum antibiotics for treatment, we implemented a two-tiered approach for treatment. The rearing system was treated with a nitrofurazone derivative, whereas the adult populations were treated using multi-antibiotic food pellets. The rearing system was treated for 3 weeks, and the adult population was treated for 2 weeks. After the completion of the antibiotic treatments, the biological filters of all of the medicated systems were seeded with nitrifying bacterial cultures. Upon the maturation of the rearing systems' biological filters, mortality rates returned to pre-outbreak levels. There have been no re-occurrences of the fish mortality since the completion of treatment. This epidemic provided some valuable lessons, lessons that if followed, will ensure faster response to unknown pathogens in the future.
Robert Russo; Dirck Dillehay
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Contemporary topics in laboratory animal science / American Association for Laboratory Animal Science     Volume:  44     ISSN:  1060-0558     ISO Abbreviation:  Contemp Top Lab Anim Sci     Publication Date:  2005 Mar 
Date Detail:
Created Date:  2005-03-18     Completed Date:  2005-04-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9204153     Medline TA:  Contemp Top Lab Anim Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  49-51     Citation Subset:  IM    
Department of Biology, Emory University, Atlanta, Georgia 30322, USA.
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MeSH Terms
Animal Feed
Animals, Laboratory*
Bacterial Infections / drug therapy,  veterinary*
Ecological Systems, Closed
Filtration / veterinary
Fish Diseases / drug therapy*,  epidemiology,  microbiology*
Nitrofurazone / therapeutic use*
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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