Document Detail


Use of boronic Acid nanoparticles in glycoprotein enrichment.
MedLine Citation:
PMID:  23296523     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Glyco-specific enrichment methods for mass spectrometry pretreatment are invaluable for the detection of low abundant glycoproteins or glycopeptides. For example, boronic acid can specifically interact with glycans in nonaqueous or basic aqueous solutions. Here, we describe a glyco-specific enrichment method which uses a boronic acid-functionalized "core-satellite" composite nanoparticle to isolate glycoproteins or glycopeptides from complex biological samples. Furthermore, we also demonstrate detection limit improvements and show how to evaluate the percent recovery from the glycoprotein or glycopeptide enrichment process via SDS-PAGE and (16)O/(18)O labeling strategies.
Authors:
Yawei Xu; Lijuan Zhang; Haojie Lu
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  951     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2013-01-08     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  45-55     Citation Subset:  IM    
Affiliation:
Department of Chemistry and Institute of Biomedical Sciences, Fudan University, Shanghai, China.
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