| Use of the BacT/Alert blood culture system for culture of sterile body fluids other than blood. | |
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MedLine Citation:
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PMID: 9774578 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Studies have demonstrated that large-volume culture methods for sterile body fluids other than blood increase recovery compared to traditional plated-medium methods. BacT/Alert is a fully automated blood culture system for detecting bacteremia and fungemia. In this study, we compared culture in BacT/Alert standard aerobic and anaerobic bottles, BacT/Alert FAN aerobic and FAN anaerobic bottles, and culture on routine media for six specimen types, i.e., continuous ambulatory peritoneal dialysate (CAPD), peritoneal, amniotic, pericardial, synovial, and pleural fluids. Specimen volumes were divided equally among the three arms of the study. A total of 1,157 specimens were tested, with 227 significant isolates recovered from 193 specimens. Recovery by method was as follows: standard bottles, 186 of 227 (82%); FAN bottles, 217 of 227 (96%); and routine culture, 184 of 227 (81%). The FAN bottles recovered significantly more gram-positive cocci (P < 0.001), Staphylococcus aureus (P = 0.003), coagulase-negative staphylococci (P = 0.008), gram-negative bacilli (P < 0.001), Enterobacteriaceae (P = 0.005), and total organisms (P < 0.001) than the routine culture. There were no significant differences in recovery between the standard bottles and the routine culture. The FAN aerobic bottle recovered significantly more gram-positive cocci (P < 0.001), S. aureus isolates (P < 0.001), coagulase-negative staphyococci (P = 0.003), and total organisms (P < 0.001) than the standard aerobic bottle, while the FAN anaerobic bottle recovered significantly more gram-positive cocci (P < 0.001), S. aureus isolates (P < 0.001), Enterobacteriaceae (P = 0.03), and total organisms (P < 0.001) than the standard anaerobic bottle. For specific specimen types, significantly more isolates were recovered from the FAN bottles compared to the routine culture for synovial (P < 0.001) and CAPD (P = 0.004) fluids. Overall, the FAN bottles were superior in performance to both the standard bottles and the routine culture for detection of microorganisms from the types of sterile body fluids included in this study. |
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Authors:
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P Bourbeau; J Riley; B J Heiter; R Master; C Young; C Pierson |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of clinical microbiology Volume: 36 ISSN: 0095-1137 ISO Abbreviation: J. Clin. Microbiol. Publication Date: 1998 Nov |
Date Detail:
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Created Date: 1998-12-11 Completed Date: 1998-12-11 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505564 Medline TA: J Clin Microbiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 3273-7 Citation Subset: IM |
Affiliation:
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Geisinger Medical Center, Danville, Pennsylvania, USA. pbourbeau@smtp.geisinger.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacteria
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growth & development,
isolation & purification* Bacteria, Aerobic / growth & development, isolation & purification Bacteria, Anaerobic / growth & development, isolation & purification Bacterial Infections / diagnosis, microbiology Bacteriological Techniques* Blood / microbiology* Body Fluids / microbiology* Culture Media Diagnostic Errors Evaluation Studies as Topic Female Humans Pregnancy |
| Chemical | |
Reg. No./Substance:
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0/Culture Media |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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