| Use of automated repetitive-sequence-based PCR for rapid laboratory confirmation of nosocomial outbreaks. | |
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MedLine Citation:
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PMID: 19879293 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: Rapid and reliable diagnosis of genetic relatedness of clinical isolates in microbiologic laboratory is essential in case of nosocomial outbreak investigation. Most molecular techniques used to type microorganisms are technically demanding and time consuming. Currently repetitive-sequence-based PCR (rep-PCR) technique has been adapted to an automated format on the DiversiLab system (bioM?rieux, Marcy l'Etoile, France). Aim of this study was to compare the performance of the DiversiLab system to that of pulsed-field gel electrophoresis (PFGE) in nosocomial outbreaks. METHODS: 122 clinical isolates (28 Methicillin-resistant Staphylococcus aureus (MRSA), 26 Acinetobacter baumannii, 45 extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae and 13 ESBL-producing Klebsiella oxytoca) were investigated. 70 isolates originated from six well-documented outbreaks, 52 were non-outbreak isolates. RESULTS: Concordant results for identification of outbreak and non-outbreak MRSA, A. baumannii and ESBL-producing K. pneumoniae strains were achieved with both methods. In the outbreak of ESBL-producing K. oxytoca automated rep-PCR was slightly more discriminatory than PFGE. Rep-PCR identified investigated ESBL-producing K. oxytoca outbreak-strains as indistinguishable or closely related, showing similarity of >90%, while PFGE identified these strains as indistinguishable. CONCLUSION: Automated rep-PCR assays on the DiversiLab system were used for MRSA, A. baumannii and for the first time ESBL-producing Klebsiella spp. and proved as a rapid and reliable method for molecular analysis of nosocomial outbreaks. |
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Authors:
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A J Grisold; G Zarfel; V Strenger; G Feierl; E Leitner; L Masoud; M Hoenigl; R B Raggam; V Dosch; E Marth |
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Publication Detail:
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Type: Journal Article Date: 2009-10-29 |
Journal Detail:
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Title: The Journal of infection Volume: 60 ISSN: 1532-2742 ISO Abbreviation: J. Infect. Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2010-02-01 Completed Date: 2010-03-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7908424 Medline TA: J Infect Country: England |
Other Details:
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Languages: eng Pagination: 44-51 Citation Subset: IM |
Copyright Information:
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2009 The British Infection Society. Published by Elsevier Ltd. All rights reserved. |
Affiliation:
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Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, Universitaetsplatz 4, A-8010 Graz, Austria. andrea.grisold@medunigraz.at |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acinetobacter Infections
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diagnosis,
epidemiology,
genetics Acinetobacter baumannii / genetics, isolation & purification Bacteria / classification, genetics, isolation & purification Bacterial Infections / diagnosis* Cross Infection / diagnosis*, epidemiology DNA, Bacterial / chemistry Disease Outbreaks* Humans Klebsiella Infections / diagnosis, epidemiology, genetics Klebsiella oxytoca / genetics, isolation & purification Klebsiella pneumoniae / genetics, isolation & purification Methicillin-Resistant Staphylococcus aureus / genetics, isolation & purification Polymerase Chain Reaction / methods* Repetitive Sequences, Nucleic Acid Staphylococcal Infections / diagnosis, epidemiology |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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