Document Detail

Use of autologous serum in corneal epithelial defects post-lamellar surgery.
MedLine Citation:
PMID:  16227849     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To evaluate the efficacy of an autologous serum treatment of post-LASIK (laser in situ keratomileusis) corneal epithelial defects in a rabbit model. METHODS: Five milliliters blood samples from 10 New Zealand rabbits were obtained by venepuncture. The serum was aseptically separated and diluted with saline solution to 20%. The final preparation was placed into 3-mL bottles with ultraviolet protection and maintained at 4 degrees C. Corneas were de-epithelialized using a 7-mm optical zone marker. A 160-microm thick flap was created in both eyes of all rabbits using an automatic corneal shaper microkeratome. Right eyes were treated with serum drops 6 times per day. Left eyes were treated with preservative-free artificial tears. Vital staining of the ocular surface and the area of corneal epithelial defect was measured daily for 1 week. Rabbits were humanely euthanized at postoperative day 7, and corneas were fixed and sectioned. Hematoxylin and eosin staining and immunohistochemical analysis were performed. RESULTS: Corneas treated with autologous serum had a statistically significant increase in the epithelial healing rate compared with those treated with artificial tears. Serum-treated corneas showed significantly less terminal transferase-mediated dUTP nick-end labeling (TUNEL) staining in the interface, minimal inflammatory cell infiltration, and less induced synthesis of stromal chondroitin sulfate than did corneas treated with preservative-free artificial tears. CONCLUSIONS: Treatment with autologous serum could be an efficient way to provide essential components to the ocular surface in the treatment of post-LASIK epithelial defects. Autologous serum induces faster epithelial healing than do artificial tears, leading to (1) a decrease in keratocyte apoptosis and migration of fibroblasts and myofibroblasts in the wound site, (2) a decrease in the migration of inflammatory cells, and (3) a consequent inhibition of cytokine release. This treatment could improve long-term refractive results post-LASIK.
Salomon Esquenazi; Jiucheng He; Haydee E P Bazan; Nicolas G Bazan
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Cornea     Volume:  24     ISSN:  0277-3740     ISO Abbreviation:  Cornea     Publication Date:  2005 Nov 
Date Detail:
Created Date:  2005-10-17     Completed Date:  2006-02-07     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8216186     Medline TA:  Cornea     Country:  United States    
Other Details:
Languages:  eng     Pagination:  992-7     Citation Subset:  IM    
Department of Ophthalmology and Neuroscience Center of Excellence, LSU Health Sciences Center, New Orleans, LA 70112, USA.
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MeSH Terms
Corneal Diseases / pathology,  prevention & control*
Disease Models, Animal
Epithelium, Corneal / pathology*,  surgery
Follow-Up Studies
In Situ Nick-End Labeling
Keratomileusis, Laser In Situ / adverse effects*
Myopia / pathology,  surgery
Ophthalmic Solutions
Wound Healing / physiology
Grant Support
Reg. No./Substance:
0/Ophthalmic Solutions

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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