| Use of aurintricarboxylic acid as an inhibitor of nucleases during nucleic acid isolation. | |
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MedLine Citation:
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PMID: 410006 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Aurintricarboxylic acid (ATA) is a general inhibitor of nucleases. ATA has been shown to inhibit the following enzymes in vitro: DNAse I, RNAse A, S1 nuclease, exonuclease III, and restriction endonucleases Sal I, Bam HI, Pst I and Sma I. The observed inhibition is consistent with the proposal by Blumenthal and Landers (BBRC 55, 680, 1973) that most nucleic acid binding proteins will be sensitive to ATA. The action of ATA as a nuclease inhibitor can be used to advantage in the isolation of cellular nucleic acids. |
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Authors:
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R B Hallick; B K Chelm; P W Gray; E M Orozco |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Nucleic acids research Volume: 4 ISSN: 0305-1048 ISO Abbreviation: Nucleic Acids Res. Publication Date: 1977 Sep |
Date Detail:
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Created Date: 1977-11-30 Completed Date: 1977-11-30 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0411011 Medline TA: Nucleic Acids Res Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 3055-64 Citation Subset: IM |
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| MeSH Terms | |
Descriptor/Qualifier:
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Aurintricarboxylic Acid
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pharmacology* Chloroplasts / analysis Cyclohexanecarboxylic Acids / pharmacology* DNA / isolation & purification* DNA Restriction Enzymes / antagonists & inhibitors* Deoxyribonucleases / antagonists & inhibitors* Euglena gracilis Kinetics Methods RNA / isolation & purification* Ribonucleases / antagonists & inhibitors* |
| Chemical | |
Reg. No./Substance:
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0/Cyclohexanecarboxylic Acids; 4431-00-9/Aurintricarboxylic Acid; 63231-63-0/RNA; 9007-49-2/DNA; EC 3.1.-/Deoxyribonucleases; EC 3.1.-/Ribonucleases; EC 3.1.21.-/DNA Restriction Enzymes |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): Nucleic Acids Res ISSN: 0305-1048 ISSN: 1362-4962 |
Article Information Download PDF  Print publication date: Month: 9 Year: 1977 Volume: 4 Issue: 9 First Page: 3055 Last Page: 3064 ID: 342634 PubMed Id: 410006 |
| Use of aurintricarboxylic acid as an inhibitor of nucleases during nucleic acid isolation. | |
| R B Hallick | |
| B K Chelm | |
| P W Gray | |
| E M Orozco, Jr | |
Article Categories:
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