Document Detail


Use of attenuated total reflectance Fourier transform infrared spectroscopy to identify microbial metabolic products on carbonate mineral surfaces.
MedLine Citation:
PMID:  18502924     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This paper demonstrates the use of attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy to detect microbial metabolic products on carbonate mineral surfaces. By creating an ATR-FTIR spectral database for specific organic acids using ATR-FTIR spectroscopy we were able to distinguish metabolic acids on calcite surfaces following Escherichia coli growth. The production of these acids by E. coli was verified using high-performance liquid chromatography with refractive index detection. The development of this technique has allowed us to identify microbial metabolic products on carbonate surfaces in nutrient-limited cave environments.
Authors:
Heather A Bullen; Stuart A Oehrle; Ariel F Bennett; Nicholas M Taylor; Hazel A Barton
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2008-05-23
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  74     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-07-14     Completed Date:  2008-07-31     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4553-9     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Northern Kentucky University, Highland Heights, KY 41099, USA.
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MeSH Terms
Descriptor/Qualifier:
Acetic Acid / metabolism*
Calcium Carbonate / chemistry*
Chromatography, High Pressure Liquid
Escherichia coli / metabolism*
Pyruvic Acid / metabolism*
Spectroscopy, Fourier Transform Infrared
Grant Support
ID/Acronym/Agency:
5P20RR016481-05/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
127-17-3/Pyruvic Acid; 471-34-1/Calcium Carbonate; 64-19-7/Acetic Acid
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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