Document Detail

Use of astrocyte-microglial cocultures to examine the regulatory influence of astrocytes on microglial activation.
MedLine Citation:
PMID:  22144319     Owner:  NLM     Status:  In-Data-Review    
Microglia are the principal immune effector cells of the central nervous system (CNS). Under normal conditions, they occupy a quiescent surveillance phenotype, but following stimulation by microorganisms or inflammatory cytokines, microglia transform into highly activated migratory, phagocytic cells producing inflammatory cytokines and chemokines. Significantly, several studies have demonstrated that astrocytes attenuate microglial activation, reducing microglial adhesion, production of interleukin-12 (IL-12) and reactive oxygen species (ROS), and expression of inducible nitric oxide synthase (iNOS). In this chapter, we describe an astrocyte-microglia coculture system that can be used to investigate interactions between these two cell types. We also describe a flow cytometry approach to quantify microglial activation state, as assessed by microglial expression of cellular activation markers, including MHC class I and the Mac-1 and α4 integrins.
Jennifer V Welser; Richard Milner
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  814     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2012  
Date Detail:
Created Date:  2011-12-06     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  367-80     Citation Subset:  IM    
Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA, USA.
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