| Use of astrocyte-microglial cocultures to examine the regulatory influence of astrocytes on microglial activation. | |
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MedLine Citation:
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PMID: 22144319 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Microglia are the principal immune effector cells of the central nervous system (CNS). Under normal conditions, they occupy a quiescent surveillance phenotype, but following stimulation by microorganisms or inflammatory cytokines, microglia transform into highly activated migratory, phagocytic cells producing inflammatory cytokines and chemokines. Significantly, several studies have demonstrated that astrocytes attenuate microglial activation, reducing microglial adhesion, production of interleukin-12 (IL-12) and reactive oxygen species (ROS), and expression of inducible nitric oxide synthase (iNOS). In this chapter, we describe an astrocyte-microglia coculture system that can be used to investigate interactions between these two cell types. We also describe a flow cytometry approach to quantify microglial activation state, as assessed by microglial expression of cellular activation markers, including MHC class I and the Mac-1 and α4 integrins. |
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Authors:
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Jennifer V Welser; Richard Milner |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 814 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2012 |
Date Detail:
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Created Date: 2011-12-06 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 367-80 Citation Subset: IM |
Affiliation:
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Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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