Document Detail


Use of analogues and the substrate-sensitivity of mutants in analysis of purine uptake and breakdown in Aspergillus nidulans.
MedLine Citation:
PMID:  6025432     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Aspergillus mutants resistant to various purine analogues (purine, 8-azaguanine, 2-thioxanthine, and 2-thiouric acid) are defective in at least one step of purine uptake or breakdown. The properties of these mutants show that there are two uptake systems for purines, one which mediates the uptake of hypoxanthine, guanine, and adenine, and the other, xanthine and uric acid. Allantoinase-less strains are sensitive to the toxic effects of allantoin accumulation. They are severely inhibited when grown in the presence of naturally occurring purines. Mutant strains derived from these, resistant to naturally occurring purines, may be isolated. These are either wild-type revertants, or carry a second metabolic block in the uptake or breakdown of purines. The properties of these double mutants confirm the interpretation of the nature of the analogue-resistant mutants.
Authors:
A J Darlington; C Scazzocchio
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  93     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1967 Mar 
Date Detail:
Created Date:  1967-07-22     Completed Date:  1967-07-22     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  937-40     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Allantoin / metabolism
Aspergillus / metabolism*
Cell Membrane Permeability
Hypoxanthines / metabolism
Mutation
Purines / metabolism*
Uric Acid / metabolism
Chemical
Reg. No./Substance:
0/Hypoxanthines; 0/Purines; 69-93-2/Uric Acid; 97-59-6/Allantoin
Comments/Corrections

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