Document Detail


Use of RAPD-PCR fingerprinting to detect genetic diversity of soil Streptomyces isolates.
MedLine Citation:
PMID:  10190117     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Random amplified polymorphic DNA (RAPD) was used for identification and assessment of genetic diversity between isolates of Streptomyces from soil. Genomic DNA from 18 Streptomyces isolates and 2 reference strains were amplified using four different 10-mer primers. Different DNA fingerprinting patterns were obtained for all the isolates. Electrophoretic and cluster analysis of the amplification products revealed incidence of polymorphism among the isolates and none of them was identical to the reference strains although there were some common amplification bands. Two highly divergent groups were determined among the isolates. The results indicate that RAPD is an efficient method for discriminating and studying genetic diversity of Streptomyces isolates.
Authors:
H I Malkawi; I Saadoun; F A Moumani; M M Meqdam
Related Documents :
15111597 - Laboratory investigation of acanthamoeba lugdunensis from patients with keratitis.
19778637 - Trypanosoma rangeli genotypes association with rhodnius prolixus and r. pallescens allo...
21177127 - Strains of staphylococcus aureus and pathology associated with chronic suppurative mast...
9569707 - Genetic diversity of ornithobacterium rhinotracheale strains isolated from poultry in f...
8144457 - Root colonization and systemic spreading of azoarcus sp. strain bh72 in grasses.
12583707 - Lactic acid bacteria isolated from dairy products inhibit genotoxic effect of 4-nitroqu...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The new microbiologica     Volume:  22     ISSN:  1121-7138     ISO Abbreviation:  New Microbiol.     Publication Date:  1999 Jan 
Date Detail:
Created Date:  1999-06-02     Completed Date:  1999-06-02     Revised Date:  2011-02-25    
Medline Journal Info:
Nlm Unique ID:  9516291     Medline TA:  New Microbiol     Country:  ITALY    
Other Details:
Languages:  eng     Pagination:  53-8     Citation Subset:  IM    
Affiliation:
Yarmouk University, Department of Biological Sciences, Irbid-Jordan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
DNA, Bacterial / analysis
Genetic Variation*
Polymorphism, Genetic
Random Amplified Polymorphic DNA Technique / classification*
Soil Microbiology*
Streptomyces / classification,  genetics*
Chemical
Reg. No./Substance:
0/DNA, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Molecular typing of Candida spp. by random amplification of polymorphic DNA and analysis of restrict...
Next Document:  An abnormally long HIV-1 env DNA PCR product due to altered sequences of primer binding sites.