Document Detail

Use of RAPD-PCR fingerprinting to detect genetic diversity of soil Streptomyces isolates.
MedLine Citation:
PMID:  10190117     Owner:  NLM     Status:  MEDLINE    
Random amplified polymorphic DNA (RAPD) was used for identification and assessment of genetic diversity between isolates of Streptomyces from soil. Genomic DNA from 18 Streptomyces isolates and 2 reference strains were amplified using four different 10-mer primers. Different DNA fingerprinting patterns were obtained for all the isolates. Electrophoretic and cluster analysis of the amplification products revealed incidence of polymorphism among the isolates and none of them was identical to the reference strains although there were some common amplification bands. Two highly divergent groups were determined among the isolates. The results indicate that RAPD is an efficient method for discriminating and studying genetic diversity of Streptomyces isolates.
H I Malkawi; I Saadoun; F A Moumani; M M Meqdam
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The new microbiologica     Volume:  22     ISSN:  1121-7138     ISO Abbreviation:  New Microbiol.     Publication Date:  1999 Jan 
Date Detail:
Created Date:  1999-06-02     Completed Date:  1999-06-02     Revised Date:  2011-02-25    
Medline Journal Info:
Nlm Unique ID:  9516291     Medline TA:  New Microbiol     Country:  ITALY    
Other Details:
Languages:  eng     Pagination:  53-8     Citation Subset:  IM    
Yarmouk University, Department of Biological Sciences, Irbid-Jordan.
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MeSH Terms
DNA, Bacterial / analysis
Genetic Variation*
Polymorphism, Genetic
Random Amplified Polymorphic DNA Technique / classification*
Soil Microbiology*
Streptomyces / classification,  genetics*
Reg. No./Substance:
0/DNA, Bacterial

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