| Use of Probemix and OmniProbe biotinylated cDNA probes for detecting HPV infection in biopsy specimens from the genital tract. | |
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MedLine Citation:
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PMID: 8391549 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIMS: To compare two commercially available pan probes for the identification of human papillomavirus (HPV) DNA expression in histological sections and to type the HPV positive cases. METHODS: 97 formalin fixed, paraffin wax embedded biopsy specimens from the genital tract were tested for HPV positivity with in situ hybridisation using biotinylated cDNA pan probes--Probemix (Enzo) and OmniProbe (Digene). The HPV positive cases were further tested with HPV types 6/11, 16/18, and 31/33/35/51, and the HPV type was related to the histological diagnosis. Formalin fixed, HeLa cells (10-50 HPV 18 copies per cell) and SiHa cells (1-2 HPV 16 copies per cell) were used as reference cell lines. RESULTS: 32% of the specimens gave positive nucleic signals with both Probemix and OmniProbe. Of these, 84% could be further characterised with regard to HPV types 6/11, 16/18, and 31/33/35/51; 4% of all cases were positive with either Probemix or OmniProbe. The concordance of these probes was high, 96% altogether. HeLa cells stained positive but SiHa cells did not. CONCLUSION: There is no difference between Probemix and OmniProbe for the general detection of HPV. The mean detection limit of these probes is about 20 copies a cell. |
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Authors:
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I Zehbe; E Rylander; A Strand; E Wilander |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of clinical pathology Volume: 46 ISSN: 0021-9746 ISO Abbreviation: J. Clin. Pathol. Publication Date: 1993 May |
Date Detail:
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Created Date: 1993-08-03 Completed Date: 1993-08-03 Revised Date: 2010-03-24 |
Medline Journal Info:
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Nlm Unique ID: 0376601 Medline TA: J Clin Pathol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 437-40 Citation Subset: AIM; IM |
Affiliation:
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Department of Pathology and Cytology, University Hospital, Uppsala, Sweden. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult DNA Probes, HPV / diagnostic use* Evaluation Studies as Topic Female Genital Diseases, Female / diagnosis* Humans In Situ Hybridization Middle Aged Papillomaviridae* / classification Sensitivity and Specificity Tumor Virus Infections / diagnosis* |
| Chemical | |
Reg. No./Substance:
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0/DNA Probes, HPV |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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