Document Detail


Use of Multi-parameter Flow Cytometry as Tool to Monitor the Impact of Formic Acid on Saccharomyces carlsbergensis Batch Ethanol Fermentations.
MedLine Citation:
PMID:  23359009     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The use of lignocellulosic materials as substrate for bioethanol production is considered a cost-effective approach to make the biofuel production process economically sustainable. However, lignocellulosic hydrolysis releases toxic compounds such as weak acids which inhibit microorganism growth and ethanol production. In order to understand the physiological response of Saccharomyces carlsbergensis when fermenting glucose in the presence of formic acid (HF), the yeast growth was monitored by multi-parameter flow cytometry. Cytoplasmic membrane potential decreased as the HF concentration increased and as the yeast culture reached the stationary phase. However, the proportion of cells with permeabilized membrane did not increase with the HF concentration increase. The accumulation of reactive oxygen species was also monitored. Control and fermentations at low HF concentrations (<1 g/L) resulted in a high proportion of highly oxidized cells at the stationary phase. The multi-parameter flow cytometry approach proved to be a useful tool to monitor the physiological stress response of S. carlsbergensis growth and ethanol production in the presence of HF, an inhibitor present in lignocellulosic hydrolysates. The information here obtained at near real time can be used to enhance second-generation bioethanol production process efficiency.
Authors:
Cláudia Freitas; Elisabete Neves; Alberto Reis; Paula C Passarinho; Teresa Lopes da Silva
Related Documents :
23949729 - Strategies for enhancing the production of penicillin g acylase from bacillus badius: i...
10891069 - Spectral tuning of avian violet- and ultraviolet-sensitive visual pigments.
8573489 - Transfer of "pseudomonas riboflavina" (foster 1944), a gram-negative, motile rod with l...
6500519 - Isolation of acid-resistant urinary trypsin inhibitors by high performance liquid chrom...
23958859 - Chemically activated formation of organic acids in reactions of the criegee intermediat...
7549109 - Purification and properties of two chitinases from streptomyces sp. j-13-3.
21699 - Purification of an acid proteinase from aspergillus saitoi and determination of peptide...
3323609 - Regulation of renal hemodynamics by plasma amino acid and hormone concentrations.
15771459 - Synthesis and biological evaluation of 1,2,4-triazinylphenylalkylthiazolecarboxylic aci...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-29
Journal Detail:
Title:  Applied biochemistry and biotechnology     Volume:  -     ISSN:  1559-0291     ISO Abbreviation:  Appl. Biochem. Biotechnol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8208561     Medline TA:  Appl Biochem Biotechnol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Laboratório Nacional de Energia e Geologia, Unidade de Bioenergia, Estrada do Paço do Lumiar, 22, 1649-038, Lisbon, Portugal.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Involvement of valgus hindfoot deformity in hallux valgus deformity in rheumatoid arthritis.
Next Document:  Pretreatment of Chicken Feather Waste for Improved Biogas Production.