| Use of Fourier-transform infrared spectroscopy for the diagnosis of failure of transfer of passive immunity and measurement of immunoglobulin concentrations in horses. | |
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MedLine Citation:
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PMID: 17708406 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: The economic, accurate, and rapid screening of foals for failure of transfer of passive immunity (FPT) is essential to ensure timely intervention. HYPOTHESIS: Infrared (IR) spectroscopy of foal sera and pattern recognition may be used to diagnose FPT and quantify serum IgG. SAMPLES: Sera from 194 foals (24-72 hours) with serum immunoglobulin G (IgG) concentrations determined previously by radial immunodiffusion assay (RID) were used. METHODS: IR spectra were recorded for the serum samples, and the data were randomly divided into training and independent test sets, each containing both FPT-positive (IgG <400 mg/dL) and non-FPT samples. A genetic optimal region selection algorithm and linear discriminant analysis were used to partition the training spectra, and the resulting classifier was then validated by comparing the IR-predicted FPT status for each of the test samples to that provided by the RID IgG assay. A quantitative IR-based assay for IgG was developed using partial least squares (PLS) and validated by testing its ability to predict IgG concentrations. RESULTS: Specificity, sensitivity, and accuracy for the combined data were 92.5, 96.8, and 95.9%, respectively. Corresponding positive (88.1%) and negative predictive (98.0%) values determined a success rate of 95-97% as compared to RID-based IgG concentrations. The IR-based quantitative assay yielded correlation coefficients for IR spectroscopy versus RID-based IgG concentrations of 0.90 and 0.86 for the training and test sets, respectively. CONCLUSIONS AND CLINICAL IMPORTANCE: The overall performance of the IR-based test was similar to that of the colorimetric assay and was superior and more economic than other available tests. |
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Authors:
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Christopher B Riley; J T McClure; Sarah Low-Ying; R Anthony Shaw |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of veterinary internal medicine / American College of Veterinary Internal Medicine Volume: 21 ISSN: 0891-6640 ISO Abbreviation: J. Vet. Intern. Med. Publication Date: 2007 Jul-Aug |
Date Detail:
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Created Date: 2007-08-21 Completed Date: 2007-09-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8708660 Medline TA: J Vet Intern Med Country: United States |
Other Details:
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Languages: eng Pagination: 828-34 Citation Subset: IM |
Affiliation:
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National Research Council of Canada, Institute for Biodiagnostics, Winnipeg, MB. criley@upei.ca |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn Horse Diseases / diagnosis* Horses Immunity, Maternally-Acquired* Immunoglobulin G / blood* Sensitivity and Specificity Spectroscopy, Fourier Transform Infrared / economics, veterinary* |
| Chemical | |
Reg. No./Substance:
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0/Immunoglobulin G |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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