Document Detail


Use of FTIR and mass spectrometry for characterization of glycated caseins.
MedLine Citation:
PMID:  19121240     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study investigates the potential of Fourier transform infrared spectroscopy (FTIR) to monitor glycation-induced changes in protein structure. Aqueous solutions of sodium caseinate and glucose (1:2 w/w, pH 6.7) were heated at 90 degrees C for 0, 10, 20, 40 and 60 min. Evidence for caseinate glycation was obtained by mass spectrometry techniques (electrospray (ESI) and matrix-assisted laser desorption ionisation (MALDI)). FTIR was able to discriminate between glycated and non-glycated sodium caseinate, when the data were analysed by multivariate statistical methods; principal component analysis (PCA) and soft independent modelling of class analogy (SIMCA). The techniques used were complementary and provided different levels of information about the glycated samples.
Authors:
Christine M Oliver; Ashwini Kher; Don McNaughton; Mary Ann Augustin
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-01-05
Journal Detail:
Title:  The Journal of dairy research     Volume:  76     ISSN:  0022-0299     ISO Abbreviation:  J. Dairy Res.     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-01-20     Completed Date:  2009-03-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985125R     Medline TA:  J Dairy Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  105-10     Citation Subset:  IM    
Affiliation:
School of Chemistry, Monash University, Clayton, VIC 3800, Australia. Christine1.Olivier@csiro.au
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MeSH Terms
Descriptor/Qualifier:
Caseins / chemistry*
Food Technology*
Mass Spectrometry / methods*
Spectroscopy, Fourier Transform Infrared / methods*
Chemical
Reg. No./Substance:
0/Caseins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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