| Use of extracellular medium chain length polyhydroxyalkanoate depolymerase for targeted binding of proteins to artificial poly[(3-hydroxyoctanoate)-co-(3-hydroxyhexanoate)] granules. | |
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MedLine Citation:
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PMID: 19459673 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Polyhydroxyalkanoates (PHA), which are produced by many microorganisms, are promising polymers for biomedical applications due to their biodegradability and biocompatibility. In this study, we evaluated the suitability of medium chain length (mcl) PHA as surface materials for immobilizing proteins. Self-stabilized, artificial mcl-PHA beads with a size of 200-300 nm were fabricated. Five of six tested proteins adsorbed nonspecifically to mcl-PHA beads in amounts of 0.4-1.8 mg m(-2) bead surface area. The binding capacity was comparable to similar-sized polystyrene particles commonly used for antibody immobilization in clinical diagnostics. A targeted immobilization of fusion proteins was achieved by using inactive extracellular PHA depolymerase (ePHA(mcl)) from Pseudomonas fluorescens as the capture ligand. The N-terminal part of ePhaZ(MCL) preceding the catalytic domain was identified to comprise the substrate binding domain and was sufficient for mediating the binding of fusion proteins to mcl-PHA. We suggest mcl-PHA to be prime candidates for both nonspecific and targeted immobilization of proteins in applications such as drug delivery, protein microarrays, and protein purification. |
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Authors:
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Julian Ihssen; David Magnani; Linda Thöny-Meyer; Qun Ren |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-05-21 |
Journal Detail:
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Title: Biomacromolecules Volume: 10 ISSN: 1526-4602 ISO Abbreviation: Biomacromolecules Publication Date: 2009 Jul |
Date Detail:
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Created Date: 2011-01-04 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100892849 Medline TA: Biomacromolecules Country: United States |
Other Details:
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Languages: eng Pagination: 1854-64 Citation Subset: IM |
Affiliation:
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Laboratory of Biomaterials, Swiss Federal Laboratories for Materials Testing and Research (EMPA), CH-9014 St. Gallen, Switzerland. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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