| Use of DNA hybridization test for diagnosing bacterial vaginosis in women with symptoms suggestive of infection. | |
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MedLine Citation:
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PMID: 17078859 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The purpose of this study was to evaluate a DNA hybridization test (Affirm VPIII) as an alternative to Gram stain for the rapid diagnosis of bacterial vaginosis in women with clinical signs of vaginal infection. Vaginal specimens were collected from 321 symptomatic women, and analyzed for bacterial vaginosis by both Gram stain using Nugent criteria and DNA hybridization test. Sensitivity, specificity, positive predictive value, and negative predictive value of the DNA hybridization test were determined using the Gram staining as the standard for diagnosis of bacterial vaginosis. Of the 321 patients, 115 (35.8%) were Gram positive for bacterial vaginosis and 126 (39.2%) were negative. 80 patients (25.0%) demonstrated intermediate Gram staining that was also considered negative. The Affirm system detected G. vaginalis in 107 (93.0%) of 115 vaginal specimens positive for bacterial vaginosis diagnosed by Gram stain. Compared to the Gram stain, DNA hybridization test had a sensitivity of 87.7% and a specificity of 96.0%. Positive and negative predictive values of the DNA hybridization test were 93.0% and 92.7%, respectively. In conclusion, Affirm VPIII hybridization test correlated well with Gram stain and may be used as a rapid diagnostic tool to exclude bacterial vaginosis in women with genital complaints. |
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Authors:
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Horu Gazi; Kenan Degerli; Ozgur Kurt; Asli Teker; Yildiz Uyar; Husnu Caglar; Semra Kurutepe; Suheyla Surucuoglu |
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Publication Detail:
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Type: Evaluation Studies; Journal Article |
Journal Detail:
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Title: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica Volume: 114 ISSN: 0903-4641 ISO Abbreviation: APMIS Publication Date: 2006 Nov |
Date Detail:
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Created Date: 2006-11-02 Completed Date: 2007-01-11 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8803400 Medline TA: APMIS Country: Denmark |
Other Details:
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Languages: eng Pagination: 784-7 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Clinical Microbiology, Celal Bayar University, School of Medicine, Manisa, Turkey. horugazi@hotmail.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult DNA, Bacterial / analysis* Female Gardnerella vaginalis / isolation & purification Gram-Negative Bacteria / isolation & purification Gram-Positive Bacteria / isolation & purification Humans Middle Aged Nucleic Acid Hybridization / methods* Sensitivity and Specificity Vaginosis, Bacterial / diagnosis* |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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