| Use of a DNA-based test for the mutation associated with porcine stress syndrome (malignant hyperthermia) in 10,000 breeding swine. | |
| | |
MedLine Citation:
|
PMID: 7901188 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
To test the hypothesis that the mutation associated with porcine stress syndrome (PSS; malignant hyperthermia) was present in a large proportion of North American and English swine, a simple and rapid laboratory protocol was used for cost-effective, large-scale diagnosis of susceptibility to PSS. This PSS test was applied to 10,245 breeding swine of various breeds from 129 farms in the United States, Canada, and England. Approximately 1 of 5 swine was a heterozygous carrier of the PSS mutation, with approximately 1% being homozygotes. Prevalence of the PSS mutation was 97% for 58 Pietrain, 35% for 1,962 Landrace, 15% for 718 Duroc, 19% for 720 Large White, 14% for 496 Hampshire, 19% for 1,727 Yorkshire, and 16% for 3,446 crossbred swine. The PPS gene frequencies for these breeds were 0.72, 0.19, 0.08, 0.10, 0.07, 0.10, and 0.09, respectively. In addition to these breeds, we have identified the PSS mutation in Poland China and Berkshire breeds. These gene frequencies were 30 to 75% lower in Canadian swine than in US swine, with the exception of Yorkshires, for which the gene frequency was threefold higher in Canadian swine. English swine were similarly, or more so, affected than were US swine. Accuracy was estimated at > 99%. Cost to perform the test was < $20/animal. Depending on the perceived net balance of deleterious and beneficial effects of the mutation, the PSS test could be used to eradicate the PSS mutation from herds, or for controlled expression of the mutation. |
| | |
Authors:
|
P J O'Brien; H Shen; C R Cory; X Zhang |
Related Documents
:
|
12580788 - Genetic relationship between mongolian and norwegian horses? 10442978 - Genetic diversity analysis of six spanish native cattle breeds using microsatellites. 8678828 - Natural scrapie in a closed flock of cheviot sheep occurs only in specific prp genotypes. 8856808 - Separating the environmental and genetic factors that may be causes of bovine spongifor... 22035158 - Novel plakophilin2 mutation: three-generation family with arrhythmogenic right ventricu... 19199618 - Surface-enhanced raman scattering based ligase detection reaction. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of the American Veterinary Medical Association Volume: 203 ISSN: 0003-1488 ISO Abbreviation: J. Am. Vet. Med. Assoc. Publication Date: 1993 Sep |
Date Detail:
|
Created Date: 1993-12-01 Completed Date: 1993-12-01 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 7503067 Medline TA: J Am Vet Med Assoc Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 842-51 Citation Subset: IM |
Affiliation:
|
Department of Pathology, Ontario Veterinary College, University of Guelph, Canada. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Base Sequence Breeding Calcium Channels / genetics* Canada / epidemiology Costs and Cost Analysis DNA / chemistry* DNA Primers / chemistry Electrophoresis, Agar Gel / economics, veterinary England / epidemiology Heterozygote Homozygote Malignant Hyperthermia / epidemiology, genetics, veterinary* Molecular Sequence Data Point Mutation* Polymerase Chain Reaction / economics, veterinary Polymorphism, Restriction Fragment Length Prevalence Specimen Handling / economics, veterinary Swine Swine Diseases / epidemiology, genetics* United States / epidemiology |
| Chemical | |
Reg. No./Substance:
|
0/Calcium Channels; 0/DNA Primers; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Expression of immediate early genes after treatment of human astrocytoma cells with radiation and ta...
Next Document: Comparative immunohistochemical studies of p53 and proliferating cell nuclear antigen expression and...