Document Detail

Use of DAPI for anastomosis group typing of strains of the fungus Rhizoctonia solani.
MedLine Citation:
PMID:  7578595     Owner:  NLM     Status:  MEDLINE    
Strains of Rhizoctonia solani, a common soil-borne, pathogenic fungus of plants, are assigned to one of 11 anastomosis groups (AGs) based on the occurrence of imperfect fusions (anastomoses) between hyphae of a non-typed strain and a tester strain of one of the 11 AG's. Imperfect fusion is characterized by the death of one or more cells in each of the hyphae involved in the fusion. Although hyphae from branches of the same strain of R. solani may fuse with each other (self-fusion), cell death does not occur. Cell death is accompanied by nuclear degradation and granulation, or plasmolysis of the cytoplasm, which often is not visible using brightfield microscopy. When the DNA-binding fluorochrome DAPI (4',6-diamidino-2-phenylindole) is used and the hyphal fusions viewed under fluorescence microscopy, no nuclei are observed in fused hyphal cells from two strains of the same AG of R. solani. Because DAPI reacts only with living nuclei, lack of staining is presumptive evidence that the fused cells are dead as a result of imperfect fusion. The use of DAPI reduces the time required for making AG determinations compared to standard methods because it eliminates the need to assess cell wall dissolution and cytoplasmic fusion. Also, it is not necessary to trace the hyphae involved in the fusion to their respective origins to ensure that self-fusion has not occurred.
M M Kulik; P D Dery
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biotechnic & histochemistry : official publication of the Biological Stain Commission     Volume:  70     ISSN:  1052-0295     ISO Abbreviation:  Biotech Histochem     Publication Date:  1995 Mar 
Date Detail:
Created Date:  1995-11-30     Completed Date:  1995-11-30     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  9107378     Medline TA:  Biotech Histochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  95-8     Citation Subset:  IM    
United States Department of Agriculture, Agricultural Research Service, Beltsville, Maryland 20705-2350, USA.
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MeSH Terms
Cell Death
Cell Nucleus
Fluorescent Dyes*
Mycological Typing Techniques
Rhizoctonia / classification*,  metabolism
Reg. No./Substance:
0/Fluorescent Dyes; 0/Indoles; 47165-04-8/DAPI

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