| Useof anti-phosphohistone H3 immunohistochemistry to determine mitotic rate in thin melanoma. | |
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MedLine Citation:
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PMID: 20559123 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The seventh edition of the American Joint Committee on Cancer (AJCC) melanoma staging system, slated for release in 2010, will introduce mitotic rate (MR) as one of the primary criteria for staging thin melanoma (< or = 1.0 mm). Accurate counts are essential because the finding of a single mitotic figure (MF) will alter the staging and management of these patients. The traditional manner of counting of mitotic figures (MFs) using a X40 objective is time consuming and prone to inter- and intraobserver variability. We employed an antibody to phosphohistone H3 (pHH3, ser10) that labels MFs in all stages of mitosis, to evaluate mitotic counts at X20 in tissue sections from 30 melanoma patients with thin lesions 0.45 to 1.2 mm in depth, and compared results with routine hematoxylin and eosin (H&E) in a double-blind fashion. The mean MR was 1.63 by antipHH3, and 0.67 for H&E, representing a mean increase of 243%. The Spearman correlation coefficient for MR in H&E and anti-pHH3 sections was 0.88 (P < 0.0001). When melanomas were designated as "mitotically active," if the MR by anti-pHH3 was > or = 2 and > or = 1 by H&E, the correlation coefficient increased to 1.0. No thin melanomas were mitotically inactive on anti-pHH3 but active on H&E. Results indicate that anti-pHH3 is a useful immunostain for labeling melanocytes in mitosis. Subsequent studies will be needed confirm the accuracy of this staining technique, which has the potential to be used as a screening method for counting MFs before conventional H&E methodology in the microstaging of thin melanoma. |
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Authors:
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David J Casper; Kate I Ross; Jane L Messina; Vernon K Sondak; Cheryl N Bodden; Tim W McCardle; L Frank Glass |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The American Journal of dermatopathology Volume: 32 ISSN: 1533-0311 ISO Abbreviation: Am J Dermatopathol Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-27 Completed Date: 2011-01-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7911005 Medline TA: Am J Dermatopathol Country: United States |
Other Details:
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Languages: eng Pagination: 650-4 Citation Subset: IM |
Affiliation:
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Department of Dermatology, University of South Florida, Tampa, FL, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Histones
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metabolism* Humans Immunohistochemistry / methods* Melanoma / metabolism, pathology* Mitosis Mitotic Index / methods Neoplasm Staging / methods* Skin Neoplasms / metabolism, pathology* |
| Chemical | |
Reg. No./Substance:
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0/Histones |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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