Document Detail


Use of an Actinobacillus pleuropneumoniae multiple mutant as a vaccine that allows differentiation of vaccinated and infected animals.
MedLine Citation:
PMID:  16790786     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Vaccination against Actinobacillus pleuropneumoniae is hampered by the lack of vaccines inducing reliable cross-serotype protection. In contrast, pigs surviving natural infection are at least partially protected from clinical symptoms upon reinfection with any serotype. Thus, we set out to construct an attenuated A. pleuropneumoniae live vaccine allowing the differentiation of vaccinated from infected animals (the DIVA concept) by successively deleting virulence-associated genes. Based on an A. pleuropneumoniae serotype 2 prototype live negative marker vaccine (W. Tonpitak, N. Baltes, I. Hennig-Pauka, and G.-F. Gerlach, Infect. Immun. 70:7120-7125, 2002), genes encoding three enzymes involved in anaerobic respiration and the ferric uptake regulator Fur were deleted, resulting in a highly attenuated sixfold mutant; this mutant was still able to colonize the lower respiratory tract and induced a detectable immune response. Upon a single aerosol application, this mutant provided significant protection from clinical symptoms upon heterologous infection with an antigenically distinct A. pleuropneumoniae serotype 9 challenge strain and allowed the serological discrimination between infected and vaccinated groups.
Authors:
Alexander Maas; Ilse D Jacobsen; Jochen Meens; Gerald-F Gerlach
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Infection and immunity     Volume:  74     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-06-22     Completed Date:  2006-08-24     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4124-32     Citation Subset:  IM    
Affiliation:
Institut fuer Mikrobiologie, Zentrum fuer Infektionsmedizin, Stiftung Tierärztliche Hochschule Hannover, Bischofsholer Damm 15, 30173 Hannover, Germany.
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MeSH Terms
Descriptor/Qualifier:
Actinobacillus Infections / immunology,  prevention & control*,  veterinary
Actinobacillus pleuropneumoniae / genetics*,  immunology*,  pathogenicity
Animals
Bacterial Proteins / physiology
Bacterial Vaccines / administration & dosage,  immunology*
Humans
Mutation*
Repressor Proteins / physiology
Swine / immunology*,  microbiology
Vaccines, Attenuated / administration & dosage,  immunology
Virulence
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Bacterial Vaccines; 0/Repressor Proteins; 0/Vaccines, Attenuated; 0/ferric uptake regulating proteins, bacterial
Comments/Corrections

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