Document Detail


Use of acid treatment and a selective medium to enhance the recovery of Francisella tularensis from water.
MedLine Citation:
PMID:  21803910     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Francisella tularensis has been associated with naturally occurring waterborne outbreaks and is also of interest as a potential biological weapon. Recovery of this pathogen from water using cultural methods is challenging due to the organism's fastidious growth requirements and interference by indigenous bacteria. A 15-min acid treatment procedure prior to culture on a selective agar was evaluated for recovery of F. tularensis from seeded water samples. Mean levels of reduction of virulent strains of F. tularensis subsp. holarctica and subsp. tularensis were less than 20% following acid treatment. The attenuated live vaccine strain (LVS) was less resistant to acid exposure. The acid treatment procedure coupled with plating on cystine heart agar with rabbit blood and antibiotics (CHARBab) allowed the isolation of F. tularensis seeded into five natural water samples.
Authors:
B W Humrighouse; N J Adcock; E W Rice
Publication Detail:
Type:  Evaluation Studies; Journal Article     Date:  2011-07-29
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  77     ISSN:  1098-5336     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-09-09     Completed Date:  2011-12-21     Revised Date:  2013-06-28    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6729-32     Citation Subset:  IM    
Affiliation:
Pegasus Technical Services Inc., Cincinnati, Ohio, USA. vkk7@cdc.gov
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MeSH Terms
Descriptor/Qualifier:
Acids / metabolism*
Animals
Antibodies, Bacterial / diagnostic use
Bacteriological Techniques / methods*
Culture Media / chemistry*
Francisella tularensis / isolation & purification*
Rabbits
Sensitivity and Specificity
Specimen Handling / methods*
Water Microbiology*
Chemical
Reg. No./Substance:
0/Acids; 0/Antibodies, Bacterial; 0/Culture Media
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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