| Use of acid treatment and a selective medium to enhance the recovery of Francisella tularensis from water. | |
| | |
MedLine Citation:
|
PMID: 21803910 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Francisella tularensis has been associated with naturally occurring waterborne outbreaks and is also of interest as a potential biological weapon. Recovery of this pathogen from water using cultural methods is challenging due to the organism's fastidious growth requirements and interference by indigenous bacteria. A 15-min acid treatment procedure prior to culture on a selective agar was evaluated for recovery of F. tularensis from seeded water samples. Mean levels of reduction of virulent strains of F. tularensis subsp. holarctica and subsp. tularensis were less than 20% following acid treatment. The attenuated live vaccine strain (LVS) was less resistant to acid exposure. The acid treatment procedure coupled with plating on cystine heart agar with rabbit blood and antibiotics (CHARBab) allowed the isolation of F. tularensis seeded into five natural water samples. |
| | |
Authors:
|
B W Humrighouse; N J Adcock; E W Rice |
Publication Detail:
|
Type: Evaluation Studies; Journal Article Date: 2011-07-29 |
Journal Detail:
|
Title: Applied and environmental microbiology Volume: 77 ISSN: 1098-5336 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2011 Sep |
Date Detail:
|
Created Date: 2011-09-09 Completed Date: 2011-12-21 Revised Date: 2013-05-23 |
Medline Journal Info:
|
Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: United States |
Other Details:
|
Languages: eng Pagination: 6729-32 Citation Subset: IM |
Affiliation:
|
Pegasus Technical Services Inc., Cincinnati, Ohio, USA. vkk7@cdc.gov |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acids
/
metabolism* Animals Antibodies, Bacterial / diagnostic use Bacteriological Techniques / methods* Culture Media / chemistry* Francisella tularensis / isolation & purification* Rabbits Sensitivity and Specificity Specimen Handling / methods* Water Microbiology* |
| Chemical | |
Reg. No./Substance:
|
0/Acids; 0/Antibodies, Bacterial; 0/Culture Media |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Novel pathway for the degradation of 2-chloro-4-nitrobenzoic acid by Acinetobacter sp. strain RKJ12.
Next Document: Response of Pseudomonas putida KT2440 to increased NADH and ATP demand.