| USDA FSIS, FDA BAM, and ISO culture methods BD BBL CHROMagar O157 media. | |
| | |
MedLine Citation:
|
PMID: 19714980 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
BBL CHROMagar O157 media (CO) was evaluated for detection of Escherichia coli O157:H7 in raw ground beef and unpasteurized apple cider. The recovery of E. coli O157:H7 on CO was compared to the U.S. Food and Drug Administration (FDA) Bacteriological Analytical Manual (BAM), U.S. Department of Agriculture (USDA) Food Safety and Inspection Service (FSIS), and International Organization for Standardization (ISO) reference-plated media using the recommended enrichment broths. Of the 180 food samples tested, 45 were tested using BAM, 45 using the USDA method, and 90 using the ISO method. CO produced comparable results with the reference methods on all matrixes with a sensitivity of 100% and a specificity of 100%. No false negatives were found in testing the food matrixes. There was no statistical difference in recovery based on Chi-square analysis. Method agreement for raw ground beef was 85% for the USDAFSIS method and 95% for the ISO method. Method agreement for unpasteurized apple cider was 100% for the ISO and FDA BAM methods. In all cases where method agreement was <100%, CO detected more positives than the reference method media. Evaluation of known isolates on CO in inclusivity and exclusivity testing had a sensitivity and specificity of 100%. The results of this study demonstrate that CO is an effective medium for the recovery and detection of E. coli O157:H7 in raw ground beef and unpasteurized apple cider using FDA BAM, USDA FSIS, and ISO methods. |
| | |
Authors:
|
Vicki Ritter; Susan Kircher; Nancy Dick |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Journal of AOAC International Volume: 92 ISSN: 1060-3271 ISO Abbreviation: J AOAC Int Publication Date: 2009 Jul-Aug |
Date Detail:
|
Created Date: 2009-08-31 Completed Date: 2009-09-24 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9215446 Medline TA: J AOAC Int Country: United States |
Other Details:
|
Languages: eng Pagination: 1118-27 Citation Subset: IM |
Affiliation:
|
BD Diagnostic Systems, 250 Schilling Circle, Hunt Valley, MD 21030, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cattle Culture Media / standards* Escherichia coli O157 / growth & development* Food Microbiology / standards* Indicators and Reagents Malus / microbiology Meat / microbiology Reagent Kits, Diagnostic Reference Standards Reproducibility of Results United States United States Department of Agriculture United States Food and Drug Administration |
| Chemical | |
Reg. No./Substance:
|
0/Culture Media; 0/Indicators and Reagents; 0/Reagent Kits, Diagnostic |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: USDA FSIS, FDA BAM, AOAC, and ISO culture methods BD BBL CHROMagar Listeria Media.
Next Document: Determination of aflatoxins and ochratoxin A in high-sugar-content traditional Turkish foods by affi...