Document Detail

USDA FSIS, FDA BAM, AOAC, and ISO culture methods BD BBL CHROMagar Listeria Media.
MedLine Citation:
PMID:  19714979     Owner:  NLM     Status:  MEDLINE    
BBL CHROMagar Listeria Media (CL) was evaluated for detection of Listeria monocytogenes in raw ground beef, smoked salmon, lettuce, and Brie cheese. The recovery of L. monocytogenes on CL was compared to the U.S. Food and Drug Administration (FDA) Bacteriological Analytical Manual (BAM), U.S. Department of Agriculture (USDA) Food Safety and Inspection Service (FSIS), AOAC, and International Organization for Standardization (ISO) reference-plated media using the recommended pre-enrichments and selective enrichments. Of the 265 food samples tested, 140 were tested using BAM, USDA, or AOAC methods and 125 were tested using ISO methods. CL produced comparable results with the reference methods on all matrixes with a sensitivity of 99.3% and a specificity of 100%. No false negatives were found in testing the food matrixes. There was no statistical difference in recovery based on Chi-square analysis. Known isolates were evaluated, and CL had a sensitivity and specificity of 100%. The results of this study demonstrate that CL is an effective medium for the recovery and detection of L. monocytogenes in raw ground beef, smoked salmon, lettuce, and Brie cheese using FDA BAM, USDA FSIS, AOAC, and ISO culture methods.
Vicki Ritter; Susan Kircher; Krista Sturm; Patty Warns; Nancy Dick
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of AOAC International     Volume:  92     ISSN:  1060-3271     ISO Abbreviation:  J AOAC Int     Publication Date:    2009 Jul-Aug
Date Detail:
Created Date:  2009-08-31     Completed Date:  2009-09-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9215446     Medline TA:  J AOAC Int     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1105-17     Citation Subset:  IM    
BD Diagnostic Systems, 250 Schilling Circle, Hunt Valley, MD 21030, USA.
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MeSH Terms
Cheese / microbiology
Culture Media / standards*
Food Microbiology / standards*
Indicators and Reagents
Lettuce / microbiology
Listeria monocytogenes / growth & development*
Meat / microbiology
Reference Standards
Reproducibility of Results
United States
United States Department of Agriculture
United States Food and Drug Administration
Reg. No./Substance:
0/Culture Media; 0/Indicators and Reagents; 0/Solutions

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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