Document Detail


Urothelial cell culture: stratified urothelial sheet and three-dimensional growth of urothelial structure.
MedLine Citation:
PMID:  23097119     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Urothelial cells line the urinary tract, including the renal pelvis, ureters, bladder, superior urethra, and the central ducts of the prostate. They are highly specialized epithelial cell types possessing unique features, imparting important functional roles in the urinary system. They act as a permeability barrier and protect underlying muscle tissues from the caustic effects of urine while also expanding with bladder filling to adjust urine pressures. The multilayered urothelium is typically structured with differentiated, mature surface cells and less mature basal cells. The basal cell layer contains tissue-specific stem cells able to self-renew for the lifetime of the mammal and also produces a pool of maturing cells for tissue homeostasis. Maintaining regenerative basal cells in a culture facilitates urothelial cell growth in vitro. Additionally, epithelial-mesenchymal communication, epithelial-matrix interactions, and cytokines/growth factors are required to maintain the normal structure and function of mature urothelial cells in vitro and to induce stem cell differentiation into urothelial cells. These cultures are useful to study the biology and physiology of the urinary tract, particularly for the development of cell-based tissue engineering strategies in urology. This chapter describes methods for the isolation of urothelial cells and their maintenance in monolayer culture, and methods for the production of multilayer urothelial cell sheets and three-dimensional cocultures of urothelial and mesenchymal cells.
Authors:
Yuanyuan Zhang; Anthony Atala
Related Documents :
23106269 - The pollen tube: a soft shell with a hard core.
24753729 - Study of flow behaviors on single-cell manipulation and shear stress reduction in micro...
22940039 - In vitro cell migration and invasion assays.
23483729 - Tumor suppressor function of ezrin-radixin-moesin-binding phosphoprotein-50 through β-...
23964669 - Synthesis of fluorescent gold nanodot-liposome hybrids for detection of phospholipase c...
23205099 - Pien tze huang inhibits the proliferation of human colon carcinoma cells by arresting g...
21091789 - Cell-density-dependent changes in mitochondrial membrane potential and reactive oxygen ...
23106269 - The pollen tube: a soft shell with a hard core.
22252639 - Heparan sulfate proteoglycans as multifunctional cell regulators: cell surface receptors.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  945     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-10-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  383-99     Citation Subset:  IM    
Affiliation:
Department of Urology, Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Winston-Salem, NC, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Primary culture and propagation of human prostate epithelial cells.
Next Document:  Analysis of tissue interactions in ectodermal organ culture.