Document Detail

Urothelial CD44 facilitates Escherichia coli infection of the murine urinary tract.
MedLine Citation:
PMID:  17082640     Owner:  NLM     Status:  MEDLINE    
Escherichia coli is the most common pathogen found in urinary tract infections (UTIs), mainly affecting children and women. We report that CD44, a hyaluronic acid (HA) binding protein that mediates cell-cell and cell-matrix interactions, facilitates the interaction of E. coli with urothelial cells and thus the infection of the host. We found that CD44 is constitutively expressed on urothelial cells and that HA accumulates in E. coli-induced UTI. In CD44-deficient mice, the bacterial outgrowth was dramatically less compared with wild-type mice despite similar granulocyte influx in the bladder and in the kidney as well as comparable cytokines/chemokines levels in both genotypes. E. coli was able to bind HA, which adhered to CD44-positive tubular epithelial cells. Most importantly, the interaction of CD44 on tubular epithelial cells with HA facilitated the migration of E. coli through the epithelial monolayer. The results provide evidence that CD44 on urothelial cells facilitates E. coli UTI. Disruption of the interaction between CD44 and HA in the bladder may provide a new approach to prevent and to treat UTI.
Kasper M A Rouschop; Marc Sylva; Gwendoline J D Teske; Inge Hoedemaeker; Steven T Pals; Jan J Weening; Tom van der Poll; Sandrine Florquin
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  177     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-11-03     Completed Date:  2007-01-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7225-32     Citation Subset:  AIM; IM    
Department of Pathology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
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MeSH Terms
Antigens, CD44 / biosynthesis,  genetics,  physiology*
Bacterial Adhesion / immunology
Bacterial Translocation / immunology
Chemokines / biosynthesis
Colony Count, Microbial
Cytokines / biosynthesis
Escherichia coli Infections / genetics,  immunology*,  microbiology,  pathology
Hyaluronic Acid / physiology
Inflammation Mediators / metabolism
Kidney Tubules / microbiology
Mice, Inbred C57BL
Mice, Knockout
Neutrophil Infiltration / genetics,  immunology
Phagocytosis / genetics,  immunology
Urinary Tract Infections / genetics,  immunology*,  microbiology,  pathology
Urothelium / cytology,  immunology*,  metabolism*
Reg. No./Substance:
0/Antigens, CD44; 0/Chemokines; 0/Cytokines; 0/Inflammation Mediators; 9004-61-9/Hyaluronic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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