Document Detail

Uromodulin (Tamm-Horsfall glycoprotein): a renal ligand for lymphokines.
MedLine Citation:
PMID:  3498215     Owner:  NLM     Status:  MEDLINE    
The protein portion of the immunosuppressive glycoprotein uromodulin is identical to the Tamm-Horsfall urinary glycoprotein and is synthesized in the kidney. Evidence that the glycoproteins are the same is based on amino acid sequence identity, immunologic cross-reactivity, and tissue localization to the thick ascending limb of Henle's loop. Nucleic acid sequencing of clones for uromodulin isolated from a complementary DNA bank from human kidney predicts a protein 639 amino acids in length, including a 24--amino acid leader sequence and a cysteine-rich mature protein with eight potential glycosylation sites. Uromodulin and preparations of Tamm-Horsfall glycoprotein bind to recombinant murine interleukin-1 (rIL-1) and human rIL-1 alpha, rIL-1 beta, and recombinant tumor necrosis factor (rTNF). Uromodulin isolated from urine of pregnant women by lectin adherence is more immunosuppressive than material isolated by the original salt-precipitation protocol of Tamm and Horsfall. Immunohistologic studies demonstrate that rIL-1 and rTNF bind to the same area of the human kidney that binds to antiserum specific for uromodulin. Thus, uromodulin (Tamm-Horsfall glycoprotein) may function as a unique renal regulatory glycoprotein that specifically binds to and regulates the circulating activity of a number of potent cytokines, including IL-1 and TNF.
C Hession; J M Decker; A P Sherblom; S Kumar; C C Yue; R J Mattaliano; R Tizard; E Kawashima; U Schmeissner; S Heletky
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Science (New York, N.Y.)     Volume:  237     ISSN:  0036-8075     ISO Abbreviation:  Science     Publication Date:  1987 Sep 
Date Detail:
Created Date:  1987-10-14     Completed Date:  1987-10-14     Revised Date:  2007-03-19    
Medline Journal Info:
Nlm Unique ID:  0404511     Medline TA:  Science     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1479-84     Citation Subset:  IM    
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MeSH Terms
Amino Acid Sequence
Base Sequence
Cloning, Molecular
Enzyme-Linked Immunosorbent Assay
Glycoproteins / metabolism
Interleukin-1 / metabolism
Kidney / metabolism*
Ligands / metabolism
Lymphokines / metabolism*
Molecular Weight
Mucoproteins / analysis*,  genetics
RNA, Messenger / analysis
Recombinant Proteins / metabolism
Tumor Necrosis Factor-alpha
Reg. No./Substance:
0/Glycoproteins; 0/Interleukin-1; 0/Ligands; 0/Lymphokines; 0/Mucoproteins; 0/RNA, Messenger; 0/Recombinant Proteins; 0/Tamm-Horsfall protein; 0/Tumor Necrosis Factor-alpha

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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