Document Detail

Urokinase gene transfer augments angiogenesis in ischemic skeletal and myocardial muscle.
MedLine Citation:
PMID:  17653104     Owner:  NLM     Status:  MEDLINE    
Urokinase plasminogen activator (uPA) is required for both endogenous and vascular endothelial growth factor (VEGF)-augmented angiogenesis in normal tissues, leading us to hypothesize that uPA augmentation by gene transfer might promote angiogenesis in ischemic tissues. Overexpression of uPA was studied in rat myocardial infarction (MI) and mouse hind limb ischemia models and compared with VEGF overexpression effects. Animals were divided into control and three experimental groups (n = 6), receiving intramuscular injections of plasmids as follows: (i) control (empty vector or expressing beta-galactosidase); (ii) uPA; (iii) VEGF(165); (iv) a 1:1 mixture of uPA and VEGF(165). The capillary densities in both ischemic models were greater (P < 0.05) in tissues treated with uPA, VEGF, or a combination of both than in controls. Infarct size was reduced in hearts from uPA and VEGF experimental groups compared with controls (P < 0.05). Local overexpression of uPA induced a marked increase in the number of macrophages and myofibroblasts present within infarcts. Hind limb blood flow was greater in all experimental groups by day 10 (P < 0.05). Overall, the effects of uPA and VEGF were uniformly comparable. Additional analysis revealed association of local edema with VEGF but not with uPA treatment. This study established that uPA gene therapy effectively induces functionally significant angiogenesis in models of acute MI and hind limb ischemia.
Dmitry O Traktuev; Zoya I Tsokolaeva; Alexander A Shevelev; Konstantin A Talitskiy; Victoria V Stepanova; Brian H Johnstone; Tahmina M Rahmat-Zade; Alexander N Kapustin; Vsevolod A Tkachuk; Keith L March; Yelena V Parfyonova
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2007-07-24
Journal Detail:
Title:  Molecular therapy : the journal of the American Society of Gene Therapy     Volume:  15     ISSN:  1525-0016     ISO Abbreviation:  Mol. Ther.     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-10-19     Completed Date:  2008-01-11     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  100890581     Medline TA:  Mol Ther     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1939-46     Citation Subset:  IM    
Indiana Center for Vascular Biology and Medicine, Indiana University, Indianapolis, Indiana, USA.
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MeSH Terms
Body Weight
Cell Movement
Gene Expression
Gene Therapy*
Hindlimb / metabolism
Ischemia / enzymology*,  genetics,  pathology*,  therapy
Leukocytes, Mononuclear / cytology
Models, Animal
Myocardium / enzymology*,  pathology*
Neovascularization, Pathologic / enzymology*
Plasmids / genetics
Urokinase-Type Plasminogen Activator / genetics,  metabolism*
Vascular Endothelial Growth Factor A / genetics,  metabolism
Reg. No./Substance:
0/VEGFA protein, human; 0/Vascular Endothelial Growth Factor A; EC Plasminogen Activator

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