Document Detail


Urine sample used for congenital toxoplasmosis diagnosis by PCR.
MedLine Citation:
PMID:  8880481     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The diagnosis of toxoplasmosis in congenitally infected infants can be difficult; serology is unreliable, and diagnosis must be based on the combination of symptomatology and direct demonstration of the parasite. Four infants suspected of having Toxoplasma gondii infection were studied by serological analysis, tissue culture, and PCR determination. T. gondii was isolated from the urine of one patient. The parasite was detected by PCR in the blood and cerebrospinal fluid of three infants and in the urine in all patients. Because nested PCR proved to be a sensitive, relatively rapid, and specific method and because it can be applied to a variety of different clinical samples, PCR can be a valuable technique for the identification of T. gondii infections in children. The present study indicates that PCR examination of urine, a fluid never before used for diagnosis in this age group, may be valuable in diagnosing cases of congenital toxoplasmosis.
Authors:
I Fuentes; M Rodriguez; C J Domingo; F del Castillo; T Juncosa; J Alvar
Publication Detail:
Type:  Case Reports; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  34     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  1996 Oct 
Date Detail:
Created Date:  1997-01-28     Completed Date:  1997-01-28     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2368-71     Citation Subset:  IM    
Affiliation:
Servicio de Parasitología, Instituto de Salud Carlos III, Madrid, Spain.
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MeSH Terms
Descriptor/Qualifier:
Animals
Female
Humans
Infant, Newborn
Polymerase Chain Reaction / methods*
Pregnancy
Sensitivity and Specificity
Toxoplasma / genetics*
Toxoplasmosis, Congenital / diagnosis*,  urine
Comments/Corrections

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