| Urine-based detection of intestinal tight junction loss. | |
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MedLine Citation:
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PMID: 19525861 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Tight junction breakdown, with loss of the important sealing protein claudin-3, is an early event in the development of intestinal damage. Therefore, noninvasive analysis of intestinal tight junction status could be helpful in early detection of intestinal injury. AIM: To investigate the usefulness of urinary claudin-3 as marker for intestinal tight junction loss. METHODS: A rat hemorrhagic shock model and a human setting of known intestinal damage, that is, patients with relapsed inflammatory bowel disease (IBD), were used to investigate intestinal tight junction status by immunohistochemical staining and urinary claudin-3 levels by western blot. RESULTS: In rats claudin-3 urine levels increased rapidly after histologically proven intestinal tight junction loss, with significantly elevated levels at 90 minutes after shock compared with sham-operated animals [mean+/-SEM: 611+/-101 intensity (INT), n=6 vs. 232+/-30 INT, n=6; P<0.05]. Moreover, in colonic biopsies of patients with IBD relapse claudin-3 staining was reduced compared with biopsies of patients with IBD without signs of disease. Concomitantly, significantly increased claudin-3 urine levels were found in these patients (502+/-67 INT, n=10) compared with patients with IBD in remission (219+/-17 INT, n=10, P<0.001) and healthy volunteers (225+/-38 INT, n=10, P<0.001). CONCLUSION: Here we show for the first time in both an experimental and clinical setting a strong relation between intestinal tight junction loss and urinary claudin-3 levels. These findings suggest that measurement of urinary claudin-3 can be used as noninvasive marker for intestinal tight junction loss. This offers new opportunities for early diagnosis and follow-up of intestinal injury. |
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Authors:
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Geertje Thuijls; Joep P M Derikx; Jacco-Juri de Haan; Joep Grootjans; Adriaan de Bru?ne; Ad A M Masclee; Erik Heineman; Wim A Buurman |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of clinical gastroenterology Volume: 44 ISSN: 1539-2031 ISO Abbreviation: J. Clin. Gastroenterol. Publication Date: 2010 Jan |
Date Detail:
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Created Date: 2009-12-22 Completed Date: 2010-03-19 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7910017 Medline TA: J Clin Gastroenterol Country: United States |
Other Details:
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Languages: eng Pagination: e14-9 Citation Subset: IM |
Affiliation:
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Department of Surgery, Maastricht University Medical Centre and Nutrition and Toxicology Research Institute Maastricht, The Netherlands. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Aged Animals Biological Markers / urine Blotting, Western Child Disease Models, Animal Female Humans Inflammatory Bowel Diseases / physiopathology* Intestines / metabolism* Male Membrane Proteins / urine* Middle Aged Rats Rats, Sprague-Dawley Shock, Hemorrhagic / physiopathology Tight Junctions / metabolism* Young Adult |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/Membrane Proteins; 0/claudin 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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