Document Detail

Urine as an alternative to urethral swabs for the diagnosis of Chlamydia trachomatis in infertile males.
MedLine Citation:
PMID:  8005709     Owner:  NLM     Status:  MEDLINE    
Swabbing the urethrae of men has been the traditional approach for collecting specimens for detection of Chlamydia trachomatis. Recently, however, urine testing using enzyme immunoassay has yielded promising results. A total of 105 patients attending the Andrology Clinic at Ga Rankuwa Hospital, Medunsa were included in the study. These patients were asymptomatic and had no urethral discharge. Three endo-urethral swabs and first-catch urine were collected from each patient. The urethral swabs were used for enzyme immunoassay (EIA) (IDEIA III), tissue culture and direct immunofluorescent antibody (DFA) test (IMAGEN) to detect C. trachomatis. In addition about 15-30 ml of first-catch urine, or urine collected at least 2h after the previous micturition, was collected for each patient for EIA testing. Fifteen (14.3%) of 105 patients were positive on urethral swab EIA, in comparison with the DFA test in which 14 (13.3%) were positive. Eight (7.8%) were positive in tissue culture. Urine EIA was positive in 17 (16.2%) patients, of whom five (4.8%) were positive in urine EIA only. All EIA positive urines were confirmed by DFA. We recommend that first-catch urine or urine collected at least 2h after the previous micturition in infertile males may be considered a suitable alternative to urethral swab for chlamydial diagnosis because it is noninvasive and nontraumatic.
T N Ramuthaga; M S Bornman; M F Mahomed; D Boomker; A S Greef; H H Crewe-Brown; S Reif
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of andrology     Volume:  17     ISSN:  0105-6263     ISO Abbreviation:  Int. J. Androl.     Publication Date:  1994 Feb 
Date Detail:
Created Date:  1994-07-19     Completed Date:  1994-07-19     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8000141     Medline TA:  Int J Androl     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  9-12     Citation Subset:  IM    
Department of Microbiological Pathology, Medical University of Southern Africa.
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MeSH Terms
Carrier State
Chlamydia Infections / complications,  diagnosis*,  epidemiology,  urine*
Chlamydia trachomatis / isolation & purification*
Evaluation Studies as Topic
Fluorescent Antibody Technique
Immunoenzyme Techniques
Infertility, Male / complications*
Middle Aged
Urethra / microbiology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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