Document Detail


Urinary trypsin inhibitor suppresses the production of interstitial procollagenase/proMMP-1 and prostromelysin 1/proMMP-3 in human uterine cervical fibroblasts and chorionic cells.
MedLine Citation:
PMID:  9409746     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The mechanisms by which urinary trypsin inhibitor (UTI) prevents preterm premature rupture of fetal membrane and premature cervical ripening were investigated. We, therefore, examined the effects of UTI on the production of matrix metalloproteinases (MMPs) which closely participate in the breakdown of extracellular matrix in cultured human uterine cervical fibroblasts and human chorionic cells. UTI suppressed specifically the production of interstitial procollagenase/proMMP-1 and prostromelysin 1/proMMP-3 from both cells in a dose-dependent manner (0.32-1.28 microM). This suppression was accompanied by a decrease in steady-state levels of their mRNAs. These results indicate for the first time that UTI down-regulates the production of proMMP-1 and proMMP-3 accompanying with the decrease in the expression of their mRNAs, and therefore UTI actually participates in the maintenance of fetal membranes and/or uterine cervix by overall suppression of MMP production along with the known inhibitory actions towards serine proteinases.
Authors:
K Imada; A Ito; N Kanayama; T Terao; Y Mori
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  FEBS letters     Volume:  417     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1997 Nov 
Date Detail:
Created Date:  1998-01-20     Completed Date:  1998-01-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  337-40     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Tokyo University of Pharmacy and Life Science, School of Pharmacy, Hachioji, Japan.
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MeSH Terms
Descriptor/Qualifier:
Blotting, Northern
Cells, Cultured
Cervix Uteri / cytology,  enzymology*
Chorion / cytology,  enzymology*
Collagenases / antagonists & inhibitors,  biosynthesis*
Enzyme Precursors / antagonists & inhibitors,  biosynthesis*
Female
Fibroblasts / enzymology
Glycoproteins / pharmacology*
Humans
Matrix Metalloproteinase 1
Metalloendopeptidases / antagonists & inhibitors,  biosynthesis*
Pregnancy
RNA, Messenger / biosynthesis
Tissue Inhibitor of Metalloproteinase-1 / biosynthesis
Transcription, Genetic / drug effects*
Trophoblasts / cytology,  enzymology
Trypsin Inhibitors / pharmacology*
Chemical
Reg. No./Substance:
0/Enzyme Precursors; 0/Glycoproteins; 0/RNA, Messenger; 0/Tissue Inhibitor of Metalloproteinase-1; 0/Trypsin Inhibitors; 80449-32-7/urinastatin; EC 3.4.24.-/Collagenases; EC 3.4.24.-/Metalloendopeptidases; EC 3.4.24.-/prostromelysin; EC 3.4.24.7/Matrix Metalloproteinase 1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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