Document Detail


Urinary prostasin in normotensive individuals: correlation with the aldosterone to renin ratio and urinary sodium.
MedLine Citation:
PMID:  23344129     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Prostasin, a glycosylphosphatidylinositol (GPI)-anchored serine protease, activates the epithelial sodium (Na) channel (ENaC), and prostasin is released in extracellular fluids, including urine. Previous data have suggested a direct association between urinary prostasin and the activation of an aldosterone-driven pathway, but a quantitative association has never been demonstrated in normotensive subjects. Similarly, physiological relationships with natriuresis or possible gender- or female hormone-related changes in urinary prostasin concentrations have never been investigated. We measured urinary prostasin by enzyme-linked immunosorbent assay in 43 healthy normotensive subjects of similar age presenting different urinary Na levels and in 15 women during the menstrual cycle and after oral estro-progestinic contraceptive (OC) therapy. Exosomal urinary prostasin was also estimated by western blotting of samples from six healthy subjects twice during the morning. Urinary prostasin presented a wide range of values (from 0.5 to 18.9 nM) without gender differences. It was positively correlated with the aldosterone to renin ratio (ARR) but not with circulating aldosterone or renin individually. Urinary prostasin was directly correlated with U-Na levels (up to 200 nmol Na), whereas it decreased for higher Na concentrations. In women, no significant changes of prostasin concentration were observed during menstrual phases. After OC therapy, prostasin increased (from 2.37±1.27 to 4.85±5.28 nM), although the increase was not statistically different (P=0.07). Prostasin was detectable in urinary exosomes and displayed a pattern similar to urinary prostasin in relation to urinary Na. In conclusion, urinary prostasin correlates with the ARR, and it is physiologically modulated by natriuresis in normotensive individuals.Hypertension Research advance online publication, 24 January 2013; doi:10.1038/hr.2012.232.
Authors:
Oliviero Olivieri; Laura Chiecchi; Francesca Pizzolo; Annalisa Castagna; Ricciarda Raffaelli; Muthukumar Gunasekaran; Patrizia Guarini; Letizia Consoli; Gianluca Salvagno; Kenichiro Kitamura
Related Documents :
25430319 - Carbon charge exchange analysis in the iter-like wall environment.
24526319 - Simultaneous determination of chromium, cadmium, and lead and evaluation of the correla...
16132719 - Different antioxidants status, total antioxidant power and free radicals in essential h...
19452319 - Study of antioxidant levels in patients with multiple myeloma.
20052549 - Effect of spinacia oleraceae l. and perilla frutescens l. on antioxidants and lipid per...
606169 - Hyperlipidaemia in children on regular haemodialysis.
12568279 - Tokishakuyakusan directly attenuates pacap's luteolytic action on luteal function in th...
8724119 - Lipoprotein(a) is increased in triglyceride-rich lipoproteins in men with coronary hear...
9822439 - At1 receptor a/c1166 polymorphism contributes to cardiac hypertrophy in subjects with h...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-24
Journal Detail:
Title:  Hypertension research : official journal of the Japanese Society of Hypertension     Volume:  -     ISSN:  1348-4214     ISO Abbreviation:  Hypertens. Res.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9307690     Medline TA:  Hypertens Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Medicine, Unit of Internal Medicine B, University of Verona, Verona, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The effect of deep vs. awake extubation on respiratory complications in high-risk children undergoin...
Next Document:  Ambulatory and home blood pressure monitoring in children and adolescents: diagnosis of hypertension...