| Urinary mRNA expression of ACE and ACE2 in human type 2 diabetic nephropathy. | |
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MedLine Citation:
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PMID: 18389211 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIMS/HYPOTHESIS: The interplay of ACE and type 2 ACE (ACE2) has been recognised as playing an important role in the tissue renin-angiotensin system within the kidney. In the present study, we measured urinary mRNA expression of ACE and ACE2 in patients with type 2 diabetic nephropathy. METHODS: We studied 50 patients with diabetic nephropathy: 26 were being treated by ACE inhibitor (ACEI) alone (ACEI group), the other 24 by ACEI and angiotensin-receptor blocker (ARB) (ACEI+ARB group). mRNA expression of ACE and ACE2 was measured by real-time quantitative RT-PCR at 0 and 12 weeks. All patients were then followed for 56 weeks. RESULTS: Proteinuria correlated significantly with urinary ACE (r=0.454, p=0.001) and ACE2 expression (r=0.651, p<0.001). Urinary ACE2 expression correlated with estimated GFR (r= -0.289, p=0.042). In the ACEI group, there was a significant inverse correlation between the rate of GFR decline and urinary ACE2 expression at baseline (r= -0.423, p=0.031) as well as at 12 weeks (r= -0.395, p=0.046). In contrast, there was no significant correlation between the rate of GFR decline and urinary ACE2 expression at baseline or at 12 weeks in the ACEI+ARB group. The rate of GFR decline did not correlate with the baseline urinary ACE expression of either group. CONCLUSION/INTERPRETATION: There was a relationship between urinary mRNA expression of ACE2 and the degree of proteinuria. The physiological implication and possibility of clinical application of quantifying urinary ACE2 expression require further study. |
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Authors:
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G Wang; F M-M Lai; K-B Lai; K-M Chow; C-H B Kwan; K-T P Li; C-C Szeto |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-04-04 |
Journal Detail:
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Title: Diabetologia Volume: 51 ISSN: 0012-186X ISO Abbreviation: Diabetologia Publication Date: 2008 Jun |
Date Detail:
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Created Date: 2008-05-01 Completed Date: 2008-08-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0006777 Medline TA: Diabetologia Country: Germany |
Other Details:
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Languages: eng Pagination: 1062-7 Citation Subset: IM |
Affiliation:
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Department of Medicine and Therapeutics, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Aged Blood Pressure DNA / genetics Diabetes Mellitus, Type 2 / enzymology*, genetics Diabetic Nephropathies / enzymology*, genetics Female Gene Expression Regulation Humans Male Middle Aged Peptidyl-Dipeptidase A / genetics*, urine* RNA, Messenger / urine* Renin-Angiotensin System / genetics Reverse Transcriptase Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 9007-49-2/DNA; EC 3.4.15.1/Peptidyl-Dipeptidase A; EC 3.4.17.-/angiotensin converting enzyme 2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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