Document Detail


Urinary mRNA expression of ACE and ACE2 in human type 2 diabetic nephropathy.
MedLine Citation:
PMID:  18389211     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIMS/HYPOTHESIS: The interplay of ACE and type 2 ACE (ACE2) has been recognised as playing an important role in the tissue renin-angiotensin system within the kidney. In the present study, we measured urinary mRNA expression of ACE and ACE2 in patients with type 2 diabetic nephropathy. METHODS: We studied 50 patients with diabetic nephropathy: 26 were being treated by ACE inhibitor (ACEI) alone (ACEI group), the other 24 by ACEI and angiotensin-receptor blocker (ARB) (ACEI+ARB group). mRNA expression of ACE and ACE2 was measured by real-time quantitative RT-PCR at 0 and 12 weeks. All patients were then followed for 56 weeks. RESULTS: Proteinuria correlated significantly with urinary ACE (r=0.454, p=0.001) and ACE2 expression (r=0.651, p<0.001). Urinary ACE2 expression correlated with estimated GFR (r= -0.289, p=0.042). In the ACEI group, there was a significant inverse correlation between the rate of GFR decline and urinary ACE2 expression at baseline (r= -0.423, p=0.031) as well as at 12 weeks (r= -0.395, p=0.046). In contrast, there was no significant correlation between the rate of GFR decline and urinary ACE2 expression at baseline or at 12 weeks in the ACEI+ARB group. The rate of GFR decline did not correlate with the baseline urinary ACE expression of either group. CONCLUSION/INTERPRETATION: There was a relationship between urinary mRNA expression of ACE2 and the degree of proteinuria. The physiological implication and possibility of clinical application of quantifying urinary ACE2 expression require further study.
Authors:
G Wang; F M-M Lai; K-B Lai; K-M Chow; C-H B Kwan; K-T P Li; C-C Szeto
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-04-04
Journal Detail:
Title:  Diabetologia     Volume:  51     ISSN:  0012-186X     ISO Abbreviation:  Diabetologia     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-01     Completed Date:  2008-08-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0006777     Medline TA:  Diabetologia     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1062-7     Citation Subset:  IM    
Affiliation:
Department of Medicine and Therapeutics, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China.
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MeSH Terms
Descriptor/Qualifier:
Aged
Blood Pressure
DNA / genetics
Diabetes Mellitus, Type 2 / enzymology*,  genetics
Diabetic Nephropathies / enzymology*,  genetics
Female
Gene Expression Regulation
Humans
Male
Middle Aged
Peptidyl-Dipeptidase A / genetics*,  urine*
RNA, Messenger / urine*
Renin-Angiotensin System / genetics
Reverse Transcriptase Polymerase Chain Reaction
Chemical
Reg. No./Substance:
0/RNA, Messenger; 9007-49-2/DNA; EC 3.4.15.1/Peptidyl-Dipeptidase A; EC 3.4.17.-/angiotensin converting enzyme 2

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