Document Detail


Urinary excretion of three nucleic acid oxidation adducts and isoprostane F(2)alpha measured by liquid chromatography-mass spectrometry in smokers, ex-smokers, and nonsmokers.
MedLine Citation:
PMID:  14607529     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To assess novel liquid chromatography/mass spectrometric methods for measuring oxidative damage to nucleic acids and lipids, we compared urinary excretion of 8-hydroxy-2'-deoxyguanosine (8-OHdG), 5-hydroxymethyl-2'-deoxyuridine (5-OHmU), and 8-hydroxyguanosine (8-OxoG), and an isoprostane, 8-iso-prostaglandin F(2)alpha (IsopF(2)alpha) in 234 healthy men (n = 113) and women (n = 121), 80 current smokers, 96 never-smokers), and 58 ex-smokers (no tobacco use for 3 years). The 8-OHdG and 8-OxoG did not differ significantly by group; 5-OHmU was higher in smokers, compared with ex- (p <.003) and never- (p <.0001) smokers and in ex- vs. never-smokers (p =.014) at, respectively, 13.5 +/- 0.7, 11.3 +/- 1.0, and 8.7 +/- 0.3 microg/g creatinine. IsopF(2)alpha was higher in smokers, compared with ex- (p =.007) and never-smokers (p <.0001) and in ex- vs. never- smokers (p =.002) at, respectively, 1.1 +/- 0.10; 0.74 +/- 0.07, and 0.51 +/- 0.04 microg/g creatinine. There were significant correlations among all three nucleic acid adducts and between IsopF(2)alpha and both 5-OHmU and 8-OHdG. Many smokers and ex-smokers had high levels of either 5-OHmU excretion or IsopF(2)alpha excretion, but not both. We conclude that 5-OHmU and IsopF(2)alpha are more discriminating of oxidative stress from tobacco smoke than the other two compounds measured. Whether characteristic patterns of excretion of these indicators forecast differential disease risk should be explored in future research.
Authors:
S Mitchell Harman; Lynn Liang; Panayiotis D Tsitouras; Frank Gucciardo; Christopher B Heward; Peter D Reaven; Wei Ping; Alaa Ahmed; Richard G Cutler
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Free radical biology & medicine     Volume:  35     ISSN:  0891-5849     ISO Abbreviation:  Free Radic. Biol. Med.     Publication Date:  2003 Nov 
Date Detail:
Created Date:  2003-11-10     Completed Date:  2004-07-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8709159     Medline TA:  Free Radic Biol Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1301-9     Citation Subset:  IM    
Affiliation:
Kronos Longevity Research Institute, Phoenix, AZ, USA. harman@kronosinstitute.org
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MeSH Terms
Descriptor/Qualifier:
Adult
Age Distribution
Aged
Aged, 80 and over
Chromatography, Liquid
DNA Adducts / metabolism*
Deoxyguanosine / analogs & derivatives*,  urine*
Dinoprost / analogs & derivatives,  urine*
Female
Guanosine / analogs & derivatives*,  urine*
Humans
Male
Mass Spectrometry
Middle Aged
Oxidation-Reduction
Oxidative Stress
Sex Distribution
Smoking / adverse effects,  metabolism*
Thymidine / analogs & derivatives*,  urine*
Chemical
Reg. No./Substance:
0/8-hydroxy-2'-deoxyguanosine; 0/DNA Adducts; 118-00-3/Guanosine; 27415-26-5/8-epi-prostaglandin F2alpha; 3868-31-3/8-hydroxyguanosine; 50-89-5/Thymidine; 5116-24-5/5-hydroxymethyl-2'-deoxyuridine; 551-11-1/Dinoprost; 961-07-9/Deoxyguanosine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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