Document Detail


Urinary excretion of the main metabolites of methamphetamine, including p-hydroxymethamphetamine-sulfate and p-hydroxymethamphetamine-glucuronide, in humans and rats.
MedLine Citation:
PMID:  16702115     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The urinary concentrations of the main metabolites of methamphetamine (MA), specifically p-hydroxymethamphetamine-sulfate (p-OHMA-Sul) and p-hydroxymethamphetamine-glucuronide (p-OHMA-Glu), were directly measured in MA users and rats using an optimized LC-ESI MS method. The concentrations of the two conjugates in 50 MA human users' urine ranged from 0.09 to 88.6 microM (0.02-21.7 microg ml-1) for p-OHMA-Sul and from <0.05 to 7.13 microM (<0.02-2.43 microg ml-1) for p-OHMA-Glu; the ratios of sulfate to glucuronide (S/G ratios) ranged from 2.2 to 37.1 (13.8+/-8.1). The results demonstrate that the sulfation is quantitatively more important than glucuronidation for the conjugation of p-OHMA in humans. The urinary concentration time-dependency in two MA users also revealed that the conjugates were mostly excreted in urine within 3 days post-intake. In contrast, in rat, almost all of the conjugated p-OHMA (>99%) was excreted as the glucuronide in urine. These findings confirm that a large species variation exists in the conjugation of p-OHMA between humans and rats.
Authors:
N Shima; H T Kamata; M Katagi; H Tsuchihashi
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Xenobiotica; the fate of foreign compounds in biological systems     Volume:  36     ISSN:  0049-8254     ISO Abbreviation:  Xenobiotica     Publication Date:    2006 Feb-Mar
Date Detail:
Created Date:  2006-05-16     Completed Date:  2006-06-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1306665     Medline TA:  Xenobiotica     Country:  England    
Other Details:
Languages:  eng     Pagination:  259-67     Citation Subset:  IM    
Affiliation:
Forensic Science Laboratory, Osaka Prefectural Police Headquarters, Osaka, Japan. nori6499@amber.plala.or.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Glucuronides / urine*
Humans
Male
Metabolic Clearance Rate
Methamphetamine / administration & dosage*,  analogs & derivatives*,  urine*
Rats
Rats, Wistar
Species Specificity
Sulfates / urine
Chemical
Reg. No./Substance:
0/Glucuronides; 0/Sulfates; 370-14-9/pholedrine; 537-46-2/Methamphetamine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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