Document Detail


Urinary excretion of 5-L-oxoproline (pyroglutamic acid) is increased in normal adults consuming vegetarian or low protein diets.
MedLine Citation:
PMID:  8914953     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method for measuring 5-L-oxoproline in urine, which involves isolation by short-column chromatography, acid hydrolysis to glutamic acid and enzymic assay of glutamic acid, was used to measure the rate of excretion in normal adults, aged 20 to 45 y. There was no difference in the daily excretion between omnivorous males (217 micromol/d) and females (195 micromol/d). In vegetarian males, urinary 5-L-oxoproline (404 micromol/d) was significantly greater than in vegetarian females (267 micromol/d, P = 0.013). Compared with omnivorous males or females, excretion of 5-L-oxoproline was significantly greater in vegetarian males (P < 0.0001) and females (P= 0.005). When normal adults consumed a diet in which the protein content was controlled at either 4.0 or 6.2 g N/d for 5 d, there was a significant increase in urinary 5-L-oxoproline on d 5, compared with either d 1 or 4. There was a significant inverse linear relationship between the increased urinary 5-L-oxoproline on the fifth dietary day and the nitrogen content of the diet. On the basis of this relationship, when the urinary excretion of 5-L-oxoproline (320 micromol/d) for vegetarians was predicted from an estimate of their dietary intake of nitrogen, the estimate was, on average, close to the measured value (345 micromol/d). As a matter of course, vegetarians excrete more 5-L-oxoproline in urine than do omnivores, and we speculated that this difference might be accounted for by differences in dietary nitrogen and the endogenous capacity for de novo synthesis of glycine.
Authors:
A A Jackson; C Persaud; T S Meakins; R Bundy
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of nutrition     Volume:  126     ISSN:  0022-3166     ISO Abbreviation:  J. Nutr.     Publication Date:  1996 Nov 
Date Detail:
Created Date:  1997-01-13     Completed Date:  1997-01-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0404243     Medline TA:  J Nutr     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2813-22     Citation Subset:  IM    
Affiliation:
Institute of Human Nutrition, University of Southampton, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aging / metabolism
Analysis of Variance
Body Height / physiology
Body Mass Index
Body Weight / physiology
Circadian Rhythm / physiology
Diet, Protein-Restricted*
Diet, Vegetarian*
Female
Glutamic Acid / analysis
Humans
Male
Middle Aged
Nitrogen / metabolism
Pyrrolidonecarboxylic Acid / urine*
Time Factors
Chemical
Reg. No./Substance:
56-86-0/Glutamic Acid; 7727-37-9/Nitrogen; 98-79-3/Pyrrolidonecarboxylic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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