| Urinary bladder membrane permeability differentially induced by membrane lipid composition. | |
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MedLine Citation:
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PMID: 19412731 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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The permeability barrier of the urothelium (covering the mammalian urinary tract) has stimulated interest in the role of the luminal membrane in the barrier function. To know how membrane lipids may affect the permeability barrier we prepare endocytic vesicles of different lipid composition entrapping a fluorescent dye (HPTS) and its quencher (DPX) using a dietary strategy (rats fed with commercial, oleic acid- or linoleic acid-enriched diets) followed by endocytosis induction. Vesicular leakage was measured by a fluorescence requenching technique. The results showed (1) endocytosed vesicles can release their content; (2) a linoleic acid-rich diet did not change either the mechanism of leakage or the amount of released material relative to the control; and (3) a oleic acid-rich diet greatly affected the mechanism of release. Thus, the dietary fatty acids can modify the urothelial cell physiology altering the pathway of endocytosed urinary fluid. |
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Authors:
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Ernesto Javier Grasso; Reyna Olga Calderón |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-05-03 |
Journal Detail:
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Title: Molecular and cellular biochemistry Volume: 330 ISSN: 1573-4919 ISO Abbreviation: Mol. Cell. Biochem. Publication Date: 2009 Oct |
Date Detail:
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Created Date: 2009-12-03 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0364456 Medline TA: Mol Cell Biochem Country: Netherlands |
Other Details:
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Languages: eng Pagination: 163-9 Citation Subset: IM |
Affiliation:
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Primera Cátedra de Biología Celular, H. y E., Instituto de Biología Celular-Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, CC220, Ciudad Universitaria, CP5000, Córdoba, Argentina. ejgrasso@hotmail.com |
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