Document Detail

Uricase-catalyzed oxidation of uric acid using an artificial electron acceptor and fabrication of amperometric uric acid sensors with use of a redox ladder polymer.
MedLine Citation:
PMID:  10361492     Owner:  NLM     Status:  MEDLINE    
Electrochemical oxidation of uric acid catalyzed by uricase (uric acid oxidase, UOx; EC was studied using several redox compounds including 5-methylphenazinium (MP) and 1-methoxy-5-methylphenazinium (MMP) as electron acceptors for UOx, which does not contain any redox cofactor. It was found that MP and MMP were useful to mediate electrons from UOx to an electrode in the enzymatic oxidation of uric acid. A novel redox polymer, poly(N-methyl-o-phenylenediamine)(poly-MPD), containing the MP units was also found to possess the mediation ability for UOx, and poly-MPD was immobilized together with UOx onto an electrode substrate covered with a self-assembled monolayer of 2-aminoethanethiolate with use of glutaraldehyde as a binding agent. The resulting electrode (poly-MPD/UOx/Au) exhibited amperometric responses to uric acid with very fast response of approximately 30 s, allowing reagentless amperometric determination in a concentration range covering that in the blood of a healthy human being. Kinetic parameters of the apparent Michaelis constant and the maximum current response obtained at the poly-MPD/UOx/Au suggested that electrochemical oxidation of uric acid was controlled by diffusion of uric acid into the enzyme film and that the redox polymer worked well in mediating between active sites of UOx molecules and the electrode substrate.
T Nakaminami; S Ito; S Kuwabata; H Yoneyama
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical chemistry     Volume:  71     ISSN:  0003-2700     ISO Abbreviation:  Anal. Chem.     Publication Date:  1999 May 
Date Detail:
Created Date:  1999-07-27     Completed Date:  1999-07-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1928-34     Citation Subset:  IM    
Department of Applied Chemistry, Faculty of Engineering, Osaka University, Japan.
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MeSH Terms
Candida / enzymology
Electron Transport
Enzymes, Immobilized / chemistry*
Urate Oxidase / chemistry*
Uric Acid / analysis*,  metabolism*
Reg. No./Substance:
0/Enzymes, Immobilized; 0/Polymers; 69-93-2/Uric Acid; EC Oxidase

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